Skip to Content
Merck

Factor XII assay with the chromogenic substrate chromozym PK.

Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie (1981-06-01)
W Dick, W Cullmann, N Müller, K Adler
ABSTRACT

A chromogenic assay for the determination of factor XII using the chromogenic substrate Chromozym PK was evaluated. The assay was linear in the range 10 U/l to more than 200 U/l. Using the assay, the normal range of factor XII (50 healthy volunteers at random) was 136 U/l +/- 27 U/l. Kallikrein-inhibiting concentrations of aprotinin did not influence factor XII. Comparison of the chromogenic with the clotting assay resulted in a correlation coefficient of r = 0.831 (p-value less than 0.001). In patients with deep vein thrombosis, factor XII level was found to be reduced to about 60% of normal activity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
N-Benzoyl-Pro-Phe-Arg-p-nitroanilide hydrochloride, protease substrate
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
175 units
Please contact Customer Service for Availability
CA$177.00
700 units
Please contact Customer Service for Availability
CA$781.00
3500 units
Please contact Customer Service for Availability
CA$3,550.00