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  • High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.

High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.

Journal of chromatography. A (2008-12-27)
Róbert Berkecz, István Ilisz, Gabriella Benedek, Ferenc Fülöp, Daniel W Armstrong, Antal Péter
ABSTRACT

The direct separation of the enantiomers of four 2-aminomono- or dihydroxycyclopentanecarboxylic acids and four 2-aminodihydroxycyclohexanecarboxylic acids was performed on chiral stationary phases containing macrocyclic glycopeptide antibiotics such as teicoplanin (Astec Chirobiotic T and T2), teicoplanin aglycone (Chirobiotic TAG) or ristocetin A (Chirobiotic R) as chiral selectors. The effects of the nature of organic modifiers, the pH, the mobile phase composition and the structures of the analytes on the separation were investigated. Chirobiotic TAG, and in some cases Chirobiotic T, proved to be the most useful of these columns. The elution sequence was determined in most cases.

MATERIALS
Product Number
Brand
Product Description

Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® HPLC Column Screening Kit, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Guard Column, Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Guard, Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 2.1 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® TAG Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® T2 Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® T Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Astec® CHIROBIOTIC® R Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm