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  • Protective effects of different antioxidants against cadmium induced oxidative damage in rat testis and prostate tissues.

Protective effects of different antioxidants against cadmium induced oxidative damage in rat testis and prostate tissues.

Systems biology in reproductive medicine (2014-04-25)
Sarwat Jahan, Asia Zahra, Umaira Irum, Natasha Iftikhar, Hizb Ullah
ABSTRACT

The present study was performed to determine the effects of different antioxidants on testicular histopathology and oxidative damage induced by cadmium (Cd) in rat testis and prostate. Twenty five rats were equally divided into five groups (n = 5/group). The control group was injected subcutaneously with saline while the Cd alone treated group received a subcutaneous injection of 0.2 mg/kg CdCl(2). Other groups were treated with sulphoraphane (25 µg/rat), vitamin E (75 mg/kg), and Ficus Religiosa plant extract (100 mg/kg) orally along with subcutaneous injections of 0.2 mg/kg CdCl(2) for fifteen days. Oxidative damage in the testicular and prostate tissues were assessed by the estimation of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), and glutathione reductase (GSR) activity. Lipid peroxidation (TBARS), protein estimation, and histomorphology were also assessed. Cadmium exposure caused a significant decrease in antioxidant enzymes like CAT, POD, SOD, GSR, protein concentrations, and a marked increase in TBARS activity in rat testis and prostate. Histological examination of adult male rat testes showed a disruption in the arrangement of seminiferous tubules along with a reduction in the number of germ cells, Leydig cells, tunica albuginea thickness, diameter of seminiferous tubules, and height of germinal epithelium. Co-treatment with vitamin E, sulphoraphane, and Ficus religiosa were found to be effective in reversing Cd induced toxicity, representing potential therapeutic options to protect the reproductive tissues from the detrimental effects of Cd toxicity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
(±)-α-Tocopherol, tested according to Ph. Eur.
Supelco
(±)-α-Tocopherol, analytical standard
Sigma-Aldrich
(±)-α-Tocopherol, synthetic, ≥96% (HPLC)
Supelco
Alpha Tocopherol, Pharmaceutical Secondary Standard; Certified Reference Material
USP
Alpha Tocopherol, United States Pharmacopeia (USP) Reference Standard
α-Tocopherol, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
α-Tocopherol, ≥95.5%
Sigma-Aldrich
(+)-α-Tocopherol, from vegetable oil, Type V, ~1000 IU/g
Sigma-Aldrich
(+)-α-Tocopherol, Type VI, from vegetable oil, liquid (≥0.88M based on potency, density and molecular wt.), BioReagent, suitable for insect cell culture, ≥1000 IU/g