Determination of testosterone esters and estradiol esters in bovine and porcine blood serum.

Determination of testosterone esters and estradiol esters in bovine and porcine blood serum.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2016-12-06)

Martina Rejtharová, Libor Rejthar, Katarína Čačková

ABSTRACT

Monitoring of steroid esters in blood serum is desirable in order to detect the possible illegal use of natural hormones as growth promoters. A method for the determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate in bovine and porcine blood serum was developed. The procedure consists of protein precipitation and removal of phospholipids using a HybridSPE®-Phospholipid column followed by clean-up on a hydrophilic modified styrene polymer Supel

MATERIALS

Product Number

Brand

Product Description

54182-U

Supelco

Supel^™-Select HLB SPE Tube, bed wt. 60 mg, volume 3 mL, pk of 50

54183-U

Supelco

Supel^™-Select HLB SPE Tube, bed wt. 200 mg, volume 6 mL, pk of 30

54181-U

Supelco

Supel^™-Select HLB SPE Tube, bed wt. 30 mg, volume 1 mL, pk of 100

55267-U

Supelco

HybridSPE^®-Phospholipid, Cartridge, bed wt. 500 mg, volume 6 mL, pk of 30