Determination of testosterone esters and nortestosterone esters in animal blood serum by LC-MS/MS.

Determination of testosterone esters and nortestosterone esters in animal blood serum by LC-MS/MS.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2017-10-06)

Martina Rejtharová, Libor Rejthar, Katarína Čačková

ABSTRACT

A sensitive and robust confirmatory method for determination of steroid esters in blood serum is essential for reliable monitoring of possible illegal use of steroid hormones as growth promoters in meat production. A previously used sample preparation methodology was improved. The procedure consists of protein precipitation and removal of phospholipids by dispersive SPE Supel™ QuE Z-Sep (Sigma-Aldrich) followed by clean-up on alumina column and LC-MS/MS measurement. The modified method has been validated according to Commission Decision 2002/657/EC. Validation parameters for determination of six testosterone esters and five nortestosterone esters in bovine and porcine blood serum are presented in this article. Decision limits for all analytes were observed in the range 10-20 pg mL

MATERIALS

Product Number

Brand

Product Description

55299-U

Supelco

Supel^™ QuE, Z-Sep+ Bulk, pkg of 20 g

55284-U

Supelco

Supel^™ QuE, Z-Sep/C18 Tube, centrifuge tube volume 2 mL, pack of 100

55411-U

Supelco