FTAQ-RO

FastStart^™ Taq DNA Polymerase, 5 U/μl

dNTPs included: no, hotstart, suitable for PCR

• Enzyme Commission number: 2.7.7.7 (BRENDA, IUBMB)
• UNSPSC Code: 41106300

Properties

• form: liquid
• Quality Level: 100
• usage: sufficient for ≤1,250 reactions (12032945001); sufficient for ≤2,500 reactions (12032953001); sufficient for 1250 reactions; sufficient for ≤250 reactions (12032929001); sufficient for 250 reactions; sufficient for 2500 reactions; sufficient for ≤50 reactions (12032902001); sufficient for 50 reactions; sufficient for ≤500 reactions (12032937001); sufficient for 500 reactions
• feature: dNTPs included: no; hotstart
• packaging: pkg of 1,000 U (12032937001 [4x250 U]); pkg of 2,500 U (12032945001 [10x250 U]); pkg of 5,000 U (12032953001 [20x250 U]); pkg of 100 U (12032902001); pkg of 500 U (12032929001 [2x250 U])
• manufacturer/tradename: Roche
• parameter: 72 °C optimum reaction temp.
• technique(s): PCR: suitable
• color: colorless
• input: purified DNA
• pH: 8-9
• solubility: water: miscible
• suitability: suitable for molecular biology
• application(s): genomic analysis; life science and biopharma
• storage temp.: −20°C

Description

General description

FastStart^™ Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart^™ Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. FastStart^™ Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.

Application

FastStart^™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR set-up and prior to the initial denaturation step.
It can be applied for:

• PCR
• Multiplex PCR
• Difficult templates e.g., secondary structures or GC-rich sequences
• Automated PCR e.g., handling at room temperatures
• Hot Start PCR up to 3kb
• Hot Start RT-PCR up to 3kb
• Quantitative reverse transcription PCR (RT-qPCR)
• Bisulfite-specific PCR

Use FastStart^™ Taq DNA Polymerase, dNTPack with ready-to-use PCR nucleotide mix.For maximum convenience, select the 2× concentrated ready-to-use FastStart^™ PCR Master.

Features and Benefits

FastStart Taq DNA Polymerase is a modified recombinant Taq DNA Polymerase, inactive at temperatures below +75°C. The kit includes an optimized PCR buffer and GC-RICH Solution for handling a wide range of templates. High enzyme stability enables pipetting by robotic stations.

• Higher specificity, sensitivity, and yield:

Hot start PCR makes PCR setup easier.

• Use robotic setup.

Use this enyzme mix stable for 24hours at +15 to +25°C.

• Prevent PCR carryover contamination.

Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes

• optimized polymerase chain reaction (PCR) buffer system and a GC-RICH solution for handling wide range of templates
• superior results due to its unique enzyme design and optimized buffer system

Packaging

1 kit containing 5 components

Quality

Each lot is function-tested using human genomic DNA and primers specific for the 365 bp fragment of human tPA gene, and, a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exo- and endonucleases and nicking activity.

Unit Definition

1 μg M13mp9ss DNA, 0.3 μg M13 sequencing primer and 0.1 μCi [α-³²P] dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Unit Assay: 1 μg M13mp9ss DNA, 0.3 μg M13 sequencing primer and 0.1 μCi [α-³²P] dCTP are incubated with varying amounts of units of FastStart Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 min. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 5 U/μl

Preparation Note

Storage conditions (working solution): Fast Start Taq mastermix is stable for 4 weeks at -25 °C to -15 °C without primers
Please note: This parameter is not part of specification

Storage and Stability

Keep container tightly closed in a dry and well-ventilated place.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent Nos. 5,677,152 and 5,773,258, and corresponding patent claims outside the US.  The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research.  No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.  This product is for research use only.  Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche.  All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany.  Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of  Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.

FastStart is a trademark of Roche

Kit Components Only

• FastStart Taq DNA Polymerase, in storage and dilution buffer 5 U/μl
• PCR Reaction Buffer, with 20 mM MgCl₂ 10x concentrated
• PCR Reaction Buffer, without MgCl₂ 10x concentrated
• MgCl₂ Stock Solution 25 mM
• GC-RICH Solution 5x concentrated

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available