FC010
Human Fibronectin
from human plasma, liquid, 1 mg/mL, purified protein, suitable for cell culture
Synonym(s):
Fibronectin protein
About This Item
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product name
Human Plasma Fibronectin Purified Protein, from human plasma, liquid, 1 mg/mL (100 MG pack size is lyophilized), purified protein, suitable for cell culture
biological source
human
Quality Level
Assay
~95% (SDS-PAGE)
form
(liquid: 1MG, 5MG, 10MG pack size
lyophilized: 100 MG pack size)
mol wt
220 kDa
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
surface coverage
2—10 μg/cm2
input
sample type mesenchymal stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type epithelial cells
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
UniProt accession no.
Binding Specificity
Peptide Source: Collagen
Peptide Source: Laminin
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
General description
Application
Biochem/physiol Actions
Physical form
Storage and Stability
Analysis Note
Other Notes
1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2.
2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.
3.Introduce the proper CO2 atmosphere, if required.
4.Add the calculated amount of HFN to each culture vessel.
5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.
6.Remove residual balanced salt solution before proceeding with standard cell culture procedures
Product is filtered using a 0.1 micron filter during production. It is recommended to filter product again before use/after reconstitution.
Legal Information
Disclaimer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
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