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C1562

Sigma-Aldrich

Freezing container, Nalgene® Mr. Frosty

H × diam. 86 mm × 117 mm

Synonym(s):

freezer container, freezer storage, storage container

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About This Item

UNSPSC Code:
41122800
NACRES:
NB.22

feature

to hold 18 x 1.0 to 2.0 mL tubes

packaging

pack of 1 ea

manufacturer/tradename

Nalgene 5100-0001

H × diam.

86 mm × 117 mm

General description

Polycarbonate container, blue high-density polyethylene closure, white high-density polyethylene vial holder, and foam insert.

Provides the critical, repeatable, 1°C/min cooling rate required for successful cryopreservation of cells. Easy to use in any mechanical freezer. Container is imprinted with graphic instructions. Requires only isopropyl alcohol. Rigid vial holder keeps vials from contacting alcohol, preventing contamination by wicking. Vial holder floats to allow thawing in a water bath. Numbers molded for identification.

All components withstand repeated freeze/thaw cycles. Stackable.

Application

Freezing container, Nalgene Mr. Frosty may be used for freezing plant cells, protoplasts , PBMC (peripheral blood mononuclear cell) .

Compatibility

Holds 18 x 1.2 and 2.0 mL cryovials

Legal Information

Nafion is a trademark of The Chemours Company FC, LLC
Nalgene is a registered trademark of Thermo Fisher Scientific or its subsidiaries

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Vanessa Cristina de Oliveira et al.
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Arend F van Peer et al.
Applied and environmental microbiology, 75(5), 1243-1247 (2008-12-31)
Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. Schizophyllum commune grows on 25 microg of phleomycin ml(-1) after
Helen A Fletcher et al.
Vaccine, 26(41), 5269-5275 (2008-08-07)
In clinical trials recombinant-modified vaccinia virus Ankara expressing the Mycobacterium tuberculosis antigen 85A (MVA85A) induces approximately 10 times more effector T cells than any other recombinant MVA vaccine. We have found that in BCG primed subjects MVA85A vaccination reduces transforming
Ji-Suk Cho et al.
Applied microbiology and biotechnology, 73(6), 1470-1476 (2006-10-06)
Transgenic suspension cells of Oryza sativa L. cv. Dongjin utilized as a host for producing recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were preserved in liquid nitrogen (-196 degrees C) after slow prefreezing in a deep freezer (-70 degrees C).
Lise M van Wijk et al.
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Recent studies have shown that the efficacy of PARP inhibitors in epithelial ovarian carcinoma (EOC) is related to tumor-specific defects in homologous recombination (HR) and extends beyond BRCA1/2 deficient EOC. A robust method with which to identify HR-deficient (HRD) carcinomas

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Protocols

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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