Skip to Content
Merck
All Photos(4)

Key Documents

CMC0012

Sigma-Aldrich

XLDNA SIG10 Electrocompetent cells

for BAC & cosmid cloning

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

biological source

Escherichia coli

grade

for molecular biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥1 x 1010 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

The XLDNA SIG10 Electrocompetent cells are designed for transformation of large constructs and increase in DNA yields.
These cells are manufactured using a novel procedure that maximizes transformation efficiencies with very large inserts and are an excellent choice for large construct transformation

Genotype

F - pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 F80dlacZΔM15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupG λ- tonA (StrR)

Features and Benefits

  • Cells that enable the most challenging library construction and screening.
  • Highest available transformation efficiencies for library construction and screening.
  • Optimized for high yields of DNA

Components

  • XLDNA SIG10 electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service