GE17-0709-10
DEAE Sepharose™ Fast Flow
Cytiva 17-0709-10, pack of 25 mL
Synonym(s):
Anion exchange purification
About This Item
Recommended Products
ligand
diethylaminoethyl
description
Ion Exchanger Type (value)
packaging
pack of 25 mL
manufacturer/tradename
Cytiva 17-0709-10
matrix
6% cross-linked agarose
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
2-12
suitability
suitable for bioprocess medium
General description
Application
As member of the BioProcess media range, DEAE Sepharose Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- Well-proven weak anion exchanger developed for industrial downstream processes
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
This page covers detailed aspects of each step in an IEX separation to improve resolution and overall performance.
Protocols
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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