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  • Identification of a tyrosine decarboxylase gene (tdcA) in Streptococcus thermophilus 1TT45 and analysis of its expression and tyramine production in milk.

Identification of a tyrosine decarboxylase gene (tdcA) in Streptococcus thermophilus 1TT45 and analysis of its expression and tyramine production in milk.

Applied and environmental microbiology (2010-12-07)
Federica La Gioia, Lucia Rizzotti, Franca Rossi, Fausto Gardini, Giulia Tabanelli, Sandra Torriani
ABSTRACT

In this study, a tyrosine decarboxylase gene (tdcA) was identified in 1 among 83 Streptococcus thermophilus strains tested. Its sequence, nearly identical to that of a tdcA of Lactobacillus curvatus, indicated a horizontal gene transfer event. Transcription in milk and the formation of critical levels of tyramine were observed in the presence of tyrosine.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Tyrosine Decarboxylase from Streptococcus faecalis, ≥0.1 unit/mg solid
Sigma-Aldrich
L-Tyrosine Decarboxylase Apoenzyme from Streptococcus faecalis, <0.005 unit/mg solid (without pyridoxal 5-phosphate), ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate)