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IPVH00010

Millipore

Immobilon® -P PVDF Membrane

1 roll, 27 cm x 3.75 m, 0.45 µm pore size, transfer membrane

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22

product name

Immobilon®-P PVDF Membrane, 1 roll, 27 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

Quality Level

400

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

27 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
 215 μg/cm2 adsorption capacity (BSA)
 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

General description

Immobilon-P transfer membrane is recommended for most Western blotting applications, especially proteins greater than 20 kDa. Immobilon-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Features and Benefits

Immobilon-P PVDF Membrane:
  • Won′t crack, curl or fracture when cut
  • Low background
  • Superior staining capabilities
  • Can be reprobed multiple times

Linkage

Replaces: INCP00010

Other Notes

Filter Code: IPVH
Primary Binding Mechanisms: Electrostatic, hydrophobic

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isabelle Robert et al.
The Journal of experimental medicine, 206(5), 1047-1056 (2009-04-15)
Immunoglobulin class switch recombination (CSR) is initiated by DNA breaks triggered by activation-induced cytidine deaminase (AID). These breaks activate DNA damage response proteins to promote appropriate repair and long-range recombination. Aberrant processing of these breaks, however, results in decreased CSR
Remodeling of VE-cadherin junctions by the human herpes virus 8 G-protein coupled receptor.
Dwyer, J, et al.
Oncogene, 30, 190-200 (2011)
Pulmonary artery smooth muscle cell senescence is a pathogenic mechanism for pulmonary hypertension in chronic lung disease.
Noureddine, Hibo, et al.
Circulation Research, 109, 543-553 (2011)
Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems.
Bourg, Gisele, et al.
Journal of Bacteriology, 191, 2985-2992 (2009)
Hao Zhang et al.
Journal of animal science and biotechnology, 11, 52-52 (2020-06-10)
Evidence indicates that early weaning predisposes piglets to intestinal oxidative stress and increases the risk of intestinal dysfunction; however, there are minimal satisfactory treatment strategies for these conditions. This study investigated the potential of resveratrol and its analog, pterostilbene, as
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Protocols

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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