00538

Chlorophyll b

analytical standard

• Empirical Formula (Hill Notation): C₅₅H₇₀MgN₄O₆
• CAS Number: 519-62-0
• Molecular Weight: 907.47
• EC Number: 208-272-4
• MDL number: MFCD00079053
• UNSPSC Code: 41116107
• NACRES: NA.24

Properties

• grade: analytical standard
• Quality Level: 100
• Assay: ≥95.0% (HPLC)
• shelf life: limited shelf life, expiry date on the label
• technique(s): HPLC: suitable; gas chromatography (GC): suitable
• application(s): cleaning products; cosmetics; food and beverages; personal care
• format: neat
• storage temp.: −20°C
• InChI: 1S/C55H71N4O6.Mg/c1-12-38-35(8)42-27-43-36(9)40(23-24-48(61)65-26-25-34(7)22-16-21-33(6)20-15-19-32(5)18-14-17-31(3)4)52(58-43)50-51(55(63)64-11)54(62)49-37(10)44(59-53(49)50)28-46-39(13-2)41(30-60)47(57-46)29-45(38)56-42;/h12,25,27-33,36,40,51H,1,13-24,26H2,2-11H3,(H-,56,57,58,59,60,62);/q-1;+2/p-1/b34-25+;/t32?,33?,36-,40-,51?;/m0./s1
• InChI key: NSMUHPMZFPKNMZ-FCFLHPMBSA-M

Description

General description

Chlorophyll b belongs to the class of porphyrins conjugated with magnesium. It absorbs light energy mostly in the blue region and along with chlorophyll a, it is responsible for the green color of plants and green algae.

Application

This analytical standard can also be used as follows:

• Fourier transform infrared (FTIR) spectroscopy-based quantification of chlorophylls (a & b) and pheophytins (a & b) from five brands of green tea samples
• Development of a high-performance liquid chromatography-diode array detection-mass spectrometric (HPLC-DAD-MS) method for the separation and determination of chlorophylls along with their derivatives in Taraxacum formosanum samples
• Analysis of 14 dietary supplement samples for the quantification of chlorophylls, its degradation products, carotenoids, and pheophytins by a UV-vis spectrophotometric method following their separation by high-performance thin-layer chromatography (HPTLC)
• Evaluation of the effect of pressure, temperature, CO₂ flow rate, and a polar co-solvent on the supercritical fluid extraction (SFE) of carotenoids and chlorophylls from microalgae Scenedesmus obliquus for use in food processing
• Simultaneous measurement of chlorophylls (a & b) and carotenoids (β-carotene and lycopene) in fruit samples of strawberry, apricot, and raspberry by two UV-vis spectrophotometric methods

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable