Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

77161

Sigma-Aldrich

Pepsin from porcine gastric mucosa

tested according to Ph. Eur.

Synonym(s):

Pepsini pulvis, Pepsin A, Pepsin from hog stomach

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9001-75-6
Enzyme Commission number:
3.4.23.1 (BRENDA, IUBMB)
EC Number:
232-629-3
MDL number:
MFCD00081840
UNSPSC Code:
12352204
eCl@ss:
42010127
NACRES:
NA.54

Agency

USP/NF
tested according to Ph. Eur.

form

powder

mol wt

35 kDa

UniProt accession no.

P00791

storage temp.

2-8°C

Gene Information

pig ... LOC396892(396892)

Looking for similar products? Visit Product Comparison Guide

Application

Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Other Notes

Sales restrictions may apply

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Heather H Pua et al.
Cell reports, 26(4), 933-944 (2019-01-24)
Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them
Liqing Zhou et al.
Behavioural brain research, 364, 374-382 (2018-01-18)
Accumulating evidence supports an increase in emotional and behavioral problems in patients with food allergy, but the underlying mechanism remains poorly understood. Here we found that in addition to inducing an increase of allergic factors in serum, food allergy also
Gao-Na Shi et al.
Therapeutic advances in medical oncology, 13, 1758835920987056-1758835920987056 (2021-02-23)
Dendritic cells (DCs) are antigen-presenting cells that play a pivotal role in adaptive cell-mediated immunity by priming and activating T cells against specific tumour and pathogenic antigens. Methotrexate (MTX), a folate derivative, functions as an immunoregulatory agent. However, the possible
Haiyu Qi et al.
Cell reports, 31(6), 107621-107621 (2020-05-14)
Follicular helper T cells (Tfhs) are essential for germinal center (GC) B cell maturation and antibody development. However, the intrinsic mechanisms that regulate Tfh differentiation are largely unknown. Here, we demonstrate that the frequencies of Tfhs and GC B cells, as
Dominik Alterauge et al.
Cell reports, 33(1), 108232-108232 (2020-10-08)
T follicular helper (Tfh) cells are crucial for the establishment of germinal centers (GCs) and potent antibody responses. Nevertheless, the T cell-intrinsic factors that are required for the maintenance of already-established Tfh cells and GCs remain largely unknown. Here, we use
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service