C2506
Cytochrome c from equine heart
≥95% (SDS-PAGE)
Synonym(s):
Cytochrome c from horse heart
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About This Item
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biological source
horse heart
Quality Level
Assay
≥95% (SDS-PAGE)
form
powder
mol wt
12,384
technique(s)
cell based assay: suitable
solubility
H2O: soluble 10 mg/mL
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
storage temp.
−20°C
Gene Information
horse ... CYCS(100053958)
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Application
Cytochrome c has been used:
- in the reduction of ferricytochrome c by xanthine oxidase-generated superoxide radicals
- as an analyte by the single droplet deposition method using MALDI mass spectra
- as a terminal electron acceptor in the assay of standard cytochrome c reductase activity
- in cytochrome c oxidase histochemistry
- in the measurement of cytochrome C oxidase activity
- as a component of phosphate buffer for the detection and evaluation of complex IV activity using blue-native gel electrophoresis (BN-PAGE)
- for detection of extracellular superoxide anion (ECSA) in isolated kidney phagocytes
The specific sites and extent of oxidation in horse cytochrome c treated with H2O2 and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.
Biochem/physiol Actions
Cytochrome c, released from the outer mitochondrial compartment into the cytosol aids in activation of caspase-9 and initiation of the apoptotic protease cascade. This process is facilitated through its interaction with apoptotic protease-activating factor-1 (APAF-1) and deoxyadenosine triphosphate (dATP).(3) Various studies suggest that measurement of cytochrome C level in serum of tumor patients can be considered as a potential marker for cell death in vivo.
Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen
Preparation Note
Prepared using TCA.
This product in solution should be dark red to reddish brown when dissolved in water at 10 mg/mL.
Other Notes
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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The Journal of biological chemistry, 266(4), 2219-2226 (1991-02-05)
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Chromatograms
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