MAK466

Free Fatty Acid Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): agriculture; food and beverages; pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): diabetes; pediatric diseases; endocrinological disorders; neurological disorders
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Fatty acids are aliphatic monocarboxylic acids ubiquitously found in animal or vegetable fat, oil, and wax. Fatty acids play important roles in cellular synthesis, energy metabolism, and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome, and Reye Syndrome.

Features and Benefits

• Linear detection range: colorimetric assay 7 - 1000 µM, fluorometric assay 7 - 100 µM fatty acid.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for detecting free fatty acids in biological samples such as serum, plasma, urine, saliva, milk, and cell cultures, and in food and agriculture products, as well as studying the effects of drugs on free fatty acid metabolism.

Principle

The Free Fatty Acid Assay Kit provides a simple, one-step and high-throughput assay for measuring Free Fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H²O². The resulting H²O² reacts with a specific dye to form a pink colored product. The optical density at 570 nm or fluorescence intensity (λEx = 530 nm/λEm = 585 nm) is directly proportional to the free fatty acid concentration in the sample.

Safety Information

• Pictograms: GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H318,H334,H410
• Precautionary Statements: P261 - P273 - P280 - P284 - P305 + P351 + P338 - P391
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2