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P0425

Sigma-Aldrich

Poly-Prep Slides

poly-L-lysine coated glass slides

Synonym(s):

Poly-L-Lysine-coated glass slides

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About This Item

UNSPSC Code:
41116124
NACRES:
NA.47

shelf life

Expiry date on the label.

application(s)

hematology
histology

storage temp.

room temp

General description

Poly-Prep slides are made of glass and designed for cell adhesion. The slides are coated with Poly-L-Lysine, a non-specific attachment factor for cells useful in promoting cell adhesion on solid substrates. Poly-L-lysine enhances binding between the cell membrane and the cultured surface.

Application

Poly-Prep Slides are used:
  • To mount liver and lung cell sections and mounted on poly-prep slides.
  • To prove better cell adhesion in clinical bronchoalveolar lavage fluid samples.
  • To determine pAkt (alpha serine/threonine-protein kinase) expression by immunohistochemistry.
  • In immunofluorescence imaging of nuclear translocation of nuclear factor κB(NFκB) in A549 cells.
  • To study the phospholipase C-zeta activity of sperm.
  • To prepare coelomocytes smears, which were further subjected to antibody staining.
Overall, glass slides coated with poly-L-lysine for tissue section adhesion in routine histological uses. And, it is not recommended for cell culture or microarray applications.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Amhed M Vargas-Velazquez et al.
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Genetic screens in the nematode Caenorhabditis elegans identified the EGF/Ras and Notch pathways as central for vulval precursor cell fate patterning. Schematically, the anchor cell secretes EGF, inducing the P6.p cell to a primary (1°) vulval fate; P6.p in turn
Clinical significance of Akt and HER2/neu overexpression in African-American and Latina women with breast cancer
Wu Y, et al.
Breast Cancer Research, 10(1), R3-R3 (2008)
The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm
Kervancioglu DE, et al.
Marmara medical journal, 629-629 (2017)
M Nizzardo et al.
Acta neuropathologica, 139(5), 837-853 (2020-02-18)
In amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA), spinal and lower brainstem motor neurons degenerate, but some motor neuron subtypes are spared, including oculomotor neurons (OMNs). The mechanisms responsible for this selective degeneration are largely unknown, but the
Pu-Ting Dong et al.
Science advances, 5(5), eaav0561-eaav0561 (2019-05-17)
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