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Key Documents

P8038

Sigma-Aldrich

Proteinase, bacterial

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

Synonym(s):

Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

type

Type XXIV

form

lyophilized powder

specific activity

≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid

mol wt

27 kDa

purified by

crystallization

shipped in

wet ice

storage temp.

−20°C

Related Categories

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.

Biochem/physiol Actions

Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Christian Czernohlavek et al.
Biointerphases, 15(1), 011002-011002 (2020-01-18)
The implementation of self-assembled biomolecules on solid materials, in particular, sensor and electrode surfaces, gains increasing importance for the design of stable functional platforms, bioinspired materials, and biosensors. The present study reports on the formation of a planar hybrid lipid/polymer
Rebecca Anna-Maria Kenngott et al.
Cells, tissues, organs, 200(2), 153-170 (2015-05-23)
In the present investigation, bovine ovary prenatal development was studied using immunohistochemistry and laser-assisted microdissection (LAM). A major aim of this study was to evaluate the protein expression pattern of intermediate filaments (IF) and distinguish S100 protein (S100 alpha and
María C Villa-Abrille et al.
American journal of physiology. Heart and circulatory physiology, 300(4), H1237-H1251 (2011-02-08)
Inhibition of Na(+)/H(+) exchanger 1 (NHE1) reduces cardiac ischemia-reperfusion (I/R) injury and also cardiac hypertrophy and failure. Although the mechanisms underlying these NHE1-mediated effects suggest delay of mitochondrial permeability transition pore (MPTP) opening, and reduction of mitochondrial-derived superoxide production, the
N V Naoumov et al.
Journal of clinical pathology, 41(7), 793-798 (1988-07-01)
A rapid and reproducible technique for in situ hybridisation, using biotin labelled probes for the Y chromosome, human DNA, hepatitis B virus DNA and cytomegalovirus DNA on formalin fixed, paraffin embedded liver tissue, was developed. The degree of proteolytic digestion
Joseph W Starnes et al.
Journal of applied physiology (Bethesda, Md. : 1985), 102(5), 1793-1798 (2007-02-17)
Exercise provides cardioprotection against ischemia-reperfusion injury, a process involving mitochondrial reactive oxygen species (ROS) generation and calcium overload. This study tested the hypotheses that isolated mitochondria from hearts of endurance-trained rats have decreased ROS production and improved tolerance against Ca(2+)-induced

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