Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

M7528

Sigma-Aldrich

Medium 199

HEPES modification, with Earle′s salts, and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Cell culture medium, M199 Medium, TCM 199

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medium 199, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

>7.2

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Marten Michaelis et al.
Andrology, 8(5), 1419-1427 (2020-04-20)
Recently we described two outbred mouse lines which have been selected for high fertility. These mouse models doubled the number of offspring per litter. Although selected for a primarily female-trait of high fertility (increased litter size), we were interested whether
Marten Michaelis et al.
BMC genomics, 18(1), 889-889 (2017-11-22)
Many genes important for reproductive performance are shared by both sexes. However, fecundity indices are primarily based on female parameters such as litter size. We examined a fertility mouse line (FL2), which has a considerably increased number of offspring and
Wenjia Li et al.
Cell death & disease, 11(7), 599-599 (2020-08-01)
Alpha-lipoic acid (α-LA), a well-known antioxidant, was proved to active ALDH2 in nitrate tolerance and diabetic animal model. However, the therapeutic advantage of α-LA for heart failure and related signaling pathway have not been explored. This study was designed to
Duo Xu et al.
Cell death & disease, 12(4), 406-406 (2021-04-17)
Escape from programmed cell death is a hallmark of cancer. In this study, we investigated the anti-apoptotic mechanisms and explored the therapeutic potential of BCL-2 homology domain-3 (BH3) mimetics in malignant pleural mesothelioma (MPM), a lethal thoracic malignancy with an
Uğur Şen et al.
Asian-Australasian journal of animal sciences, 31(6), 827-834 (2017-12-23)
The aim of this study was to compare the effects of 36.5°C and 38.5°C incubation temperatures on the maturation of bovine oocytes and developmental competence of embryos. In experiment 1, oocytes were maturated in bicarbonate-buffered TCM-199 for 22 hours in
View All Related Papers

Articles

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Related Content

Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service