Gel electrophoresis

Applied Filters:

facet applications:Gel electrophoresis

Showing 1-30 of 43 results

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.

Components of Rehydration Solution

This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Formamide Molecular Biology Reagent

Formamide is a reagent that is an ionizing solvent in aqueous buffers. It is widely utilized in biochemistry and molecular biology, particularly in nucleic acids research.

Paper-Bridge Loading

This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products

Applying Equilibrated Immobiline® DryStrip Gels

This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.

DNA / Protein Electrophoresis and Troubleshooting Tables

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Proteomic analysis of membrane proteins

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System

Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.

Winning Westerns Technique Talk: Perfecting Western Blotting

In this webinar, Alli Lynch, Ph.D. reviews the basics of Western blotting, identifies common troubleshooting issues, and provides tips for Western blotting success.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Precast Agarose Gels for RNA Electrophoresis

Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with Cytiva products.

Troubleshooting 2-D DIGE Results

Troubleshooting table for 2D DIGE results lists problems, causes, and prevention methods for future experiments.

Water for Protein Electrophoresis & Western Blotting

Explore the impact of water quality on protein electrophoresis and Western blotting. Learn about the role of ultrapure water and its effect on chemiluminescent signals in Western blotting.

Precipitation Procedures

This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Protein Visualization

Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

Preparing for IEF

This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.

Inserting Gels into Ettan™ DALT Electrophoresis Units

This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.

How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System

Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.

Rehydration Solutions

This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.

Reproducibility with Biological Buffers

Biological buffers are organic substances that maintain a constant pH over a given range by neutralizing the effects of hydrogen ions.

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

Page 1 of 2