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DNTPCA10

Sigma-Aldrich

CleanAmp™ dNTP

10 μmol of each dNTP, Modified dNTP set for hot-start PCR

Synonym(s):

Deoxynucleotides, dNTP, dNTP Mix

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About This Item

UNSPSC Code:
41106305

form

liquid

quality

10 μmol of each dNTP

feature

hotstart

concentration

50 mM (each dNTP)

color

colorless

shipped in

dry ice

storage temp.

−20°C

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General description

CleanAmp dNTPs, are a product of TriLink BioTechnologies, Inc. CleanAmp dNTPs are modified nucleoside triphosphates that block DNA polymerase nucleotide incorporation. CleanAmp dNTPs are activated by the initial heating step and subsequent denaturing steps in typical hot start PCR cycling conditions. This process limits the amount of activated dNTPs during each cycle of PCR, allowing for more specific and efficient amplification of the desired product and also reducing or completely avoiding mis-priming or primer dimer formation. CleanAmp dNTPs provide a more affordable solution than hot start enzymes for a variety of PCR-based applications.

Application

  • For qPCR amplifications using SYBR
  • For multiplex PCR
  • For reduction of primer dimers

Packaging

10 μmoles: 4 vials:
200 μL dATP at 50 mM in buffered glycine solution
200 μL dCTP at 50 mM in buffered glycine solution
200 μL dTTP at 50 mM in buffered glycine solution
200 μL dGTP at 50 mM in buffered glycine solution

Legal Information

CleanAmp™ dNTPs are provided to buyer with a non-transferrable right from TriLink BioTechnologies, Inc. to use the purchased CleanAmp™ dNTPs in internal research conducted by the buyer, whether the buyer is an academic, non-profit, or for-profit entity. CleanAmp™ dNTPs are not to be used for human therapeutic or commercial clinical diagnostic use. A license is required for any commercial use of CleanAmp™ dNTPs, regardless of the academic or non-profit status of the using entity. Information about commercial licenses for CleanAmp™ dNTPs may be obtained from TriLink BioTechnologies, Inc. Buyer may not use the CleanAmp™ dNTPs to support the filing of a patent application in any country in the world that contains claims directed to CleanAmp™ dNTPs or uses thereof without the express approval of TriLink BioTechnologies, Inc. Buyer′s right to have and use the CleanAmp™ dNTPs will terminate immediately if you fail to comply with these terms and conditions of this agreement.
CleanAmp is a trademark of TriLink BioTechnologies, Inc.

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

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JAN Code

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Certificates of Analysis (COA)

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Xinglong Xiao et al.
Journal of virological methods, 187(2), 357-361 (2012-11-13)
Nucleic acid testing (NAT) is valuable for screening blood donors for occult hepatitis B virus (HBV) infection and infection during the window period in countries where HBV is endemic, such as China. An "in-house" NAT (Triplex NAT) was developed for
Franck Court et al.
Nucleic acids research, 39(14), 5893-5906 (2011-04-12)
Parental genomic imprinting at the Igf2/H19 locus is controlled by a methylation-sensitive CTCF insulator that prevents the access of downstream enhancers to the Igf2 gene on the maternal chromosome. However, on the paternal chromosome, it remains unclear whether long-range interactions
Franck Court et al.
Genome biology, 12(5), R42-R42 (2011-05-17)
Despite its critical role for mammalian gene regulation, the basic structural landscape of chromatin in living cells remains largely unknown within chromosomal territories below the megabase scale. Here, using the 3C-qPCR method, we investigate contact frequencies at high resolution within
Elena Hidalgo Ashrafi et al.
Current protocols in molecular biology, Chapter 15, Unit 15-Unit 15 (2009-10-10)
Hot-start PCR is a technique that improves PCR performance by reducing nonspecific amplification during the initial setup stages of the PCR. This unit describes hot-start PCR protocols which utilize primers containing temperature-sensitive modifications. The introduction of 4-oxo-tetradecyl (OXT) phosphotriester groups
Inna Koukhareva et al.
Nucleic acids symposium series (2004), (52)(52), 259-260 (2008-09-09)
Several 3'-ether and 3'-ester derivatives of 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) were prepared. These dNTP derivatives were not substrates for DNA polymerase and did not support primer extension at room temperature. However, by short pre-heating to 95 degrees C in PCR buffer
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