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Key Documents

Safety Information

T8154

Sigma-Aldrich

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Blue cell viability dye

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5 G
₩142,709
25 G
₩514,402
100 G
₩1,748,845
500 G
₩3,045,609

₩142,709


Estimated to ship onJanuary 31, 2025Details



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5 G
₩142,709
25 G
₩514,402
100 G
₩1,748,845
500 G
₩3,045,609

About This Item

Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Beilstein:
4360496
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

₩142,709


Estimated to ship onJanuary 31, 2025Details


sterility

sterile-filtered

Quality Level

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable
tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

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1 of 4

This Item
D60015242331404
grade

for molecular biology

grade

-

grade

-

grade

-

technique(s)

FISH: suitable, FTIR: suitable

technique(s)

-

technique(s)

-

technique(s)

-

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL, clear, colorless to faintly greenish-yellow

solubility

H2O: 0.1 g/mL, clear, colorless to slightly yellow, water: 100 g/L at 25 °C (77 °F ), H2O: freely soluble 50 mg/mL, clear

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

200

form

powder

form

powder

form

powder

form

powder

General description

For the same product in a convenient lyophilized (powder) format, please see T6146




Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue.[1] It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.[2]
Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane.[3] This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Trypan Blue solution has been used:
  • in cell viability assay to count viable and dead cells[4]
  • in rescue assay to count viable cells[4]
  • in trypan blue exclusion counting method to determine proliferation curve[5]
  • as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion[6]
  • in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability[7]
  • to count GEC cells using a hemocytometer[7]

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Carc. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

T8154-PROC:
T8154-1LSTAB:
T8154-20ML:
T8154-VAR:
T8154-BULK:
T8154-1000ML:
T8154-100ML:


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