Key Documents
Safety Information
T8154
Trypan Blue solution
0.4%, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Blue cell viability dye
Select a Size
₩142,709
Estimated to ship onJanuary 31, 2025Details
Select a Size
About This Item
₩142,709
Estimated to ship onJanuary 31, 2025Details
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
storage condition
dry at room temperature
concentration
0.4%
technique(s)
cell culture | mammalian: suitable
tissue processing: suitable
application(s)
cell analysis
shipped in
ambient
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | D6001 | 52423 | 31404 |
---|---|---|---|
for molecular biology | - | - | - |
FISH: suitable, FTIR: suitable | - | - | - |
H2O: 100 mg/mL | H2O: 100 mg/mL | H2O: 100 mg/mL, clear, colorless to faintly greenish-yellow | H2O: 0.1 g/mL, clear, colorless to slightly yellow, water: 100 g/L at 25 °C (77 °F ), H2O: freely soluble 50 mg/mL, clear |
300 | 200 | 100 | 200 |
powder | powder | powder | powder |
General description
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue.[1] It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.[2]
Application
- in cell viability assay to count viable and dead cells[4]
- in rescue assay to count viable cells[4]
- in trypan blue exclusion counting method to determine proliferation curve[5]
- as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion[6]
- in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability[7]
- to count GEC cells using a hemocytometer[7]
Preparation Note
also commonly purchased with this product
comparable product
recommended
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
T8154-PROC:
T8154-1LSTAB:
T8154-20ML:
T8154-VAR:
T8154-BULK:
T8154-1000ML:
T8154-100ML:
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Articles
Cell counting protocol using a hemocytometer for monitoring cell viability. Discover other cell counting tools such as the Scepter™ 3.0 Cell Counter.
Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.
Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Protocols
Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.
Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.
Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.
Learn how to cultivate similar-sized iPSC-derived colon organoids using Millicell® Microwell plates and perform a forskolin-induced swelling assay.
Related Content
Three-dimensional (3D) printing of biological tissue is rapidly becoming an integral part of tissue engineering.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service