Skip to Content
Merck
All Photos(1)

Key Documents

Safety Information

21888

Sigma-Aldrich

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

CFSE

Sign Into View Organizational & Contract Pricing

Select a Size

W302406-SAMPLE-K
₩44,051
1 KG
₩155,498
10 KG
₩336,168
25 KG
₩698,422

₩44,051


Please contact Customer Service for Availability

Request a Bulk Order

Select a Size

Change View
W302406-SAMPLE-K
₩44,051
1 KG
₩155,498
10 KG
₩336,168
25 KG
₩698,422

About This Item

Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
Molecular Weight:
557.46
MDL number:
UNSPSC Code:
12352108
PubChem Substance ID:
NACRES:
NA.32

₩44,051


Please contact Customer Service for Availability

Request a Bulk Order

product line

BioReagent

Quality Level

Assay

≥90% (HPLC)

form

solid

mp

152-154 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
6143165.060715.32583
TAK-242 InSolution, ≥98%, 25 mM in DMSO, TLR4 inhibitor

5.08336

TAK-242

I-BET InSolution, ≥98%, 50 mM in DMSO, A cell-permeable benzodiazepine compound

5.06071

I-BET

form

liquid

form

solid

form

liquid

form

powder

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−70°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

storage condition

OK to freeze, desiccated (hygroscopic), avoid repeated freeze/thaw cycles, protect from light

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light

storage condition

OK to freeze, protect from light

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.[1]

Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry.[2][3] Forms green fluorescent conjugates on deacetylation.
5[4]-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
  • to label cells for flow cytometry[5][6]
  • in lymphocyte proliferation assay[7][8]
  • to determine the intracellular or intra-organellar pH in chloroplasts[4]
  • to label Leishmania amazonensis and Leishmania major[9]

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.[10]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

21888-BULK-F:
21888-VAR-F:
21888-25MG-F:
21888-100MG-F:


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

M Bronner-Fraser
The Journal of cell biology, 101(2), 610-617 (1985-08-01)
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest
WNT5A transforms intestinal CD8αα+ IELs into an unconventional phenotype with pro-inflammatory features
Zhao D, et al.
BMC Gastroenterology, 15(1), 173-173 (2015)
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe
Su PH and Lai YH
Frontiers in Plant Science, 8, 2079-2079 (2017)
Microbial Food Safety Along the Dairy Chain (2017)
Juan Dubrot et al.
The Journal of experimental medicine, 211(6), 1153-1166 (2014-05-21)
Dendritic cells (DCs), and more recently lymph node stromal cells (LNSCs), have been described to tolerize self-reactive CD8(+) T cells in LNs. Although LNSCs express MHCII, it is unknown whether they can also impact CD4(+) T cell functions. We show

Articles

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service