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D8906

Sigma-Aldrich

Dextran sulfate sodium salt from Leuconostoc spp.

for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

Synonym(s):

6-O-(6-O-beta-D-Glucopyranosyl-beta-D-glucopyranosyl)-D-glucose, Manninotriose

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200 MG
₩610,523

₩610,523


Estimated to ship onJanuary 31, 2025Details


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200 MG
₩610,523

About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352201
NACRES:
NA.31

₩610,523


Estimated to ship onJanuary 31, 2025Details


Request a Bulk Order

biological source

synthetic

Quality Level

grade

for molecular biology

form

powder

mol wt

average Mw >500,000 (dextran starting material)

contains

0.5-2% phosphate buffer

composition

Sulfur Content, 16.0-19.0%

technique(s)

FISH: suitable
FTIR: suitable

color

off-white

useful pH range

6.0-8 (10 g/L)

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This Item
D60015242331404
grade

for molecular biology

grade

-

grade

-

grade

-

technique(s)

FISH: suitable, FTIR: suitable

technique(s)

-

technique(s)

-

technique(s)

-

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL

solubility

H2O: 100 mg/mL, clear, colorless to faintly greenish-yellow

solubility

H2O: 0.1 g/mL, clear, colorless to slightly yellow, water: 100 g/L at 25 °C (77 °F ), H2O: freely soluble 50 mg/mL, clear

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

200

form

powder

form

powder

form

powder

form

powder

General description

Dextran sulfate sodium (DSS) is a synthetic sulfated derivative of dextran, a complex polysaccharide composed of glucose molecules. DSS is characterized as a biocompatible, highly branched polysaccharide with 1–6 and 1–4 glycosidic linkages, featuring approximately 2.3 sulfate groups per glucosyl unit. Dextran sulfate is a sulfated polysaccharide with a variable molecular weight ranging from 5 to 1,400 kDa. It finds extensive use in molecular biology and biochemical research, with a particular emphasis on gastrointestinal investigations.DSS is widely recognized for its ability to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD). It is widely recognized for its capacity to induce colonic inflammation in mouse models of inflammatory bowel disease (IBD) by creating models of acute, chronic, and relapsing IBD, by adjusting its concentration and administration frequency. Furthermore, Dextran sulfate is applied in various molecular biology techniques, such as precipitating lipoproteins, accelerating DNA probe hybridization, and facilitating ribosomal isolations. Notably, DSS serves as a valuable cross-linker for chitosan, allowing for the creation of microparticles and nanoparticles used in controlled drug delivery systems. Beyond these applications, DSS plays a central role in diverse research areas, particularly immunology and virology, where it actively modulates immune responses, enhances humoral immunity, influences B-lymphocyte activation, and affects thymocyte reactivity to lectins.

Application

Dextran sulfate has been used

  • as a molecular weight marker to assess the purity and homogeneity of the purified polysaccharides by gel electrophoresis[1]
  • as a component of the hybridization buffer in fluorescence in situ hybridization (FISH) protocol for DNA detection and analysis[2][3]
Dextran sulfate has many applications and when performing nucleic acid hybridizations, may be used as an accelerant to decrease the time required for probe annealing. Dextran sulfate may be included in the hybridization buffer when the rate of hybridization is a limiting factor in detecting rare sequences or it may be included in the hybridization buffer when a limited amount of probe is available. Dextran sulfate functions as an accelerant by excluding the nucleic acid from the volume of solution occupied by the polymer, thereby, increasing the effective probe concentration.

Biochem/physiol Actions

Dextran sulfate, being a sulfated polysaccharide, consistently triggers experimental acute and chronic colitis. It is believed to initiate mucosal injury and inflammation, primarily through a direct toxic impact on epithelial cells. This initial effect activates macrophages and T lymphocytes, ultimately leading to cytokine-mediated cytotoxicity.

Features and Benefits

  • Suitable for Nucleic acid hybridization & Molecular Biology
  • Dextran Sulfate with an average molecular weight of >500,000
  • Sulfur Content of 16.0-19.0 %
  • Versatile and adaptable for wide variety of laboratory and research applications

Suitability

Tested for suitability in nucleic acid hybridizations

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

D8906-500G:
D8906-BULK:
D8906-VAR:
D8906-5G:
D8906-100G:
D8906-50G:
D8906-10G:


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A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses
Fernando et al.
International Journal of Biological Macromolecules, 104, 1185-1193 (2017)
Ewoud van Tricht et al.
Talanta, 166, 8-14 (2017-02-19)
During development of adenovirus-based vaccines, samples have to be analyzed in order to either monitor the production process or control the quality and safety of the product. An important quality attribute is the total concentration of intact adenoviruses, which currently
Bhavana Tiwari et al.
Current biology : CB, 27(19), 3010-3016 (2017-10-03)
Retrotransposons are a pervasive class of mobile elements present in the genomes of virtually all forms of life [1, 2]. In metazoans, these are preferentially active in the germline, which, in turn, mounts defenses that restrain their activity [3, 4].
Danielle L Cullinane et al.
Reproduction (Cambridge, England), 149(1), 43-54 (2014-10-23)
The protamine 1 (Prm1) and sperm mitochondria-associated, cysteine-rich protein (Smcp) mRNAs exemplify a widespread pattern of mRNA-specific regulation of mRNA translation in post-meiotic spermatogenic cells, spermatids. Both mRNAs are transcribed and initially stored in free-mRNPs in early spermatids, and translated
Regulation of intestinal stem cell activity by a mitotic cell cycle regulator Polo in Drosophila
Zhang et al.
G3 (Bethesda, Md.), 16, 84-84 (2023)

Articles

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

Protocols

Dextran sulfates as sodium salts are water-soluble and stable, containing approximately 17% sulfur for various applications.

Related Content

Dextrans are polysaccharides with molecular weights ≥1,000 Dalton, featuring a linear backbone of α-linked d-glucopyranosyl repeating units.

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