The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

Journal of nanoscience and nanotechnology (2014-04-18)

Wei-Qiang Yu, Ling Xu, Fu-Qiang Zhang

ABSTRACT

The TiO2 nanotubes by anodization have been extensively studied for medical implant and orthopedic applications because of enhancing bone development. In the present study, a new nano-foveolae structure verified by SEM and AFM was prepared by simulating the nanotubes exfoliation from anodized Ti. MC3T3-E1 preosteoblasts were used to investigate the effects of new nanoscale surface on cell spreading, proliferation, and osteogenic gene expression. The new nano-foveolae structure supported preosteoblast better spreading, more filopodiaes shown by SEM (4 h), and lower proliferation (72 h) than the smooth Ti. By two weeks, the new nanoscale surface induced higher expression of osteogenic markers alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and collagen I (COL I) with real-time RT-PCR compared to the control group. The results of this study suggest that the new nano-foveolae structure provides a favorable surface for functions of bone cells.

MATERIALS

Product Number

Brand

Product Description

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

348848

Sigma-Aldrich

Titanium, foil, thickness 0.025 mm, 99.98% trace metals basis

GF34106363

Titanium, rod, 1000mm, diameter 1.5mm, annealed, 99.6+%

GF05925281

Titanium, microfoil, disks, 10mm, thinness 0.1μm, specific density 42.8μg/cm², permanent mylar 3.5μm support, 99.6+%

GF16031843

Titanium, mesh, 100x100mm, nominal aperture 0.19mm, wire diameter 0.23mm, 60x60 wires/inch, open area 20%, twill weave

GF35061083

Titanium, tube, 200mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF62977540

Titanium, tube, 200mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%

GF25290581

Titanium, tube, 1000mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

GF04311638

Titanium, rod, 100mm, diameter 6mm, annealed, 99.6+%

GF48167590

Titanium, tube, 100mm, outside diameter 6.1mm, inside diameter 5.1mm, wall thickness 0.5mm, annealed, 99.6+%

GF29391329

Titanium, tube, 1000mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF28411703

Titanium, tube, 100mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%

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Titanium, rod, 200mm, diameter 2mm, as drawn, 99.99+%

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Titanium, tube, 1000mm, outside diameter 12.7mm, inside diameter 10.9mm, wall thickness 0.9mm, annealed, 99.6+%

GF13900205

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Titanium, rod, 200mm, diameter 3.0mm, annealed, 99.6+%

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Titanium, rod, 100mm, diameter 5mm, annealed, 99.6+%

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Titanium, rod, 200mm, diameter 5mm, annealed, 99.6+%

GF14377631

Titanium, wire reel, 25m, diameter 0.25mm, annealed, 99.6+%

GF46132534

Titanium, wire reel, 100m, diameter 0.25mm, as drawn, 99.6+%

GF00761217

Titanium, wire reel, 200m, diameter 0.25mm, as drawn, 99.6+%

GF55775975

Titanium, wire reel, 25m, diameter 0.25mm, as drawn, 99.6+%