コンテンツへスキップ
Merck
  • A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly.

A Yap-dependent mechanoregulatory program sustains cell migration for embryo axis assembly.

Nature communications (2023-05-17)
Ana Sousa-Ortega, Javier Vázquez-Marín, Estefanía Sanabria-Reinoso, Jorge Corbacho, Rocío Polvillo, Alejandro Campoy-López, Lorena Buono, Felix Loosli, María Almuedo-Castillo, Juan R Martínez-Morales
要旨

The assembly of the embryo's primary axis is a fundamental landmark for the establishment of the vertebrate body plan. Although the morphogenetic movements directing cell convergence towards the midline have been described extensively, little is known on how gastrulating cells interpret mechanical cues. Yap proteins are well-known transcriptional mechanotransducers, yet their role in gastrulation remains elusive. Here we show that the double knockout of yap and its paralog yap1b in medaka results in an axis assembly failure, due to reduced displacement and migratory persistence in mutant cells. Accordingly, we identified genes involved in cytoskeletal organization and cell-ECM adhesion as potentially direct Yap targets. Dynamic analysis of live sensors and downstream targets reveal that Yap is acting in migratory cells, promoting cortical actin and focal adhesions recruitment. Our results indicate that Yap coordinates a mechanoregulatory program to sustain intracellular tension and maintain the directed cell migration for embryo axis development.

材料
製品番号
ブランド
製品内容

Sigma-Aldrich
ダサチニブ, ≥98% (HPLC)
カタログ番号
容量
在庫状況
単価
数量
Roche
Anti-Digoxigenin-POD, Fab fragments, from sheep
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
抗ホスホヒストンH3 (Ser10)抗体、有糸分裂マーカー, Upstate®, from rabbit
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
カリクリンA Discodermia calyx(海綿)由来, ≥90% (HPLC), solid
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
(-)-Blebbistatin, The active enantiomer of (±)-Blebbistatin that accounts for the inhibitory activity towards ATPase and myosin II-dependent cellular processes.
カタログ番号
容量
在庫状況
単価
数量
Sigma-Aldrich
Rho Kinase Inhibitor III, Rockout, The Rho Kinase Inhibitor III, Rockout, also referenced under CAS 7272-84-6, controls the biological activity of Rho Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.
カタログ番号
容量
在庫状況
単価
数量