N2513
Nerve Growth Factor β rat
≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
Synonym(s):
Nerve Growth Factor-β from rat
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product name
NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
biological source
rat
Quality Level
recombinant
expressed in Sf21 cells
Assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
0.15-3.00 ng/mL EC50
packaging
pkg of 0.1 mg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
solubility
water: soluble 0.1 g/L at 20 °C
UniProt accession no.
storage temp.
−20°C
Gene Information
rat ... NGF(4803)
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General description
NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells. It is a neurotrophin and belongs to the NGF family.
Application
NGF-β from rat is used for the following applications:
- In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)
- Western blotting analysis
- For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)
- In cell culture and treatment (for neuronal differentiation)
- In the preparation of stock solution
Biochem/physiol Actions
NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers. It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same. It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.
Physical form
Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine
Analysis Note
The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Guangyi Zhao et al.
Molecular medicine reports, 16(4), 4678-4684 (2017-08-30)
The present study aimed to investigate the effect of exogenous nerve growth factor (NGF) pretreatment on demyelination in the spinal cord of lidocaine‑treated rats, and explored the potential neuroprotective mechanisms of NGF. A total of 36 rats were randomly assigned
Xiao-Ping Huang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 89, 124-134 (2017-02-22)
The aim of this study was to explore the effect by which the combination of Astragaloside IV (AST IV) and Ginsenoside Rg1 (Rg1) resisted autophagic injury in PC12 cells induced by oxygen glucose deprivation/reoxygenation (OGD/R). We studied the nature of
A Khodorova et al.
Neuroscience, 254, 312-323 (2013-10-08)
Nerve growth factor (NGF) augments the excitability of isolated rat sensory neurons through activation of the p75 neurotrophin receptor (p75(NTR)) and its downstream sphingomyelin signaling cascade, wherein neutral sphingomyelinase(s) (nSMase), ceramide, and the atypical protein-kinase C (aPKC), protein-kinase M zeta
Zhe Gong et al.
Journal of neuroinflammation, 15(1), 242-242 (2018-08-30)
Nod-like receptor protein 3 (NLRP3) inflammasome is a crucial factor in mediating inflammatory responses after cerebral ischemia/reperfusion (I/R), but the cellular location of NLRP3 inflammasome in cerebral I/R has yet come to a conclusion, and there is still no specific
Niels Speksnijder et al.
Journal of molecular neuroscience : MN, 48(1), 209-218 (2012-05-25)
The glucocorticoid receptor (GR) and myocyte enhancer factor 2 (MEF2) are transcription factors involved in neuronal plasticity. c-JUN, a target gene of GR and MEF2, plays a role in regulating both synaptic strength and synapse number. The aim of this
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