ZRB1875
Anti-Cleaved Caspase-8 Antibody, clone 1K3 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
CASP-8, CASP8, EC: 3.4.22.61, FLICE, MACH, MCH5
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1K3, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 43 kDa
observed mol wt ~55 kDa
purified by
using Protein A
species reactivity
human, mouse
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
mouse ... Casp8(12370)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1K3 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects cleaved form of Caspase-8. It targets an epitope within 23 amino acids from the cleavage site within the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the cleavage site within the C-terminal half of mouse Caspase-8.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa treated with Staurosporin.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cleaved Caspase-8 in lysates from overnight serum starved HeLa cells treated with Staurosporin (0.5 M; 6 h).
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cleaved Caspase-8 in lysates from NIH3T3 cells treated with Etoposide (100 nM; 2 h).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in Mouse brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa treated with Staurosporin.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cleaved Caspase-8 in lysates from overnight serum starved HeLa cells treated with Staurosporin (0.5 M; 6 h).
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cleaved Caspase-8 in lysates from NIH3T3 cells treated with Etoposide (100 nM; 2 h).
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in Mouse brain tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Cleaved Caspase-8 in NIH3T3 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Caspase-8 (UniProt: O89110; also known as EC: 3.4.22.61, CASP-8, CASP8, MCH5, FLICE, MACH) is encoded by the Casp8 gene (Gene ID: 12370) in murine species. Caspase-8 is a cysteine protease that serves as a molecular switch for apoptosis, necroptosis, and pyroptosis, and is required to prevent tissue damage during embryonic development and adulthood. It is an initiator caspase that plays a major role in the extrinsic apoptotic signaling pathway via death receptors. It is expressed in wide variety of tissues and its highest expression has been reported in spleen, thymus, lung, liver, and kidney. Low levels of expression are observed in heart, brain, testis, and skeletal muscle. Caspase-8 is produced with pro-peptides (aa 1-218 and 377-387) that are cleaved under appropriate signals to produce a p20 (aa 219-376) and a p10 (aa 388-480) subunits that heterotetramerize to form active caspase-8 with p20 and two p10 subunits. Generation of the subunits requires association with the death-inducing signaling complex (DISC). Activated caspase 8 is subsequently released to the cytosol where it cleaves downstream effector caspases. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Newton, K., et al. (2019). Nature. 574(7778); 428-431; Fritsch, M., et al. (2019). Nature. 575(7784); 683-687).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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