- Quantification of Bacteria Residing in Caenorhabditis elegans Intestine.
Quantification of Bacteria Residing in Caenorhabditis elegans Intestine.
Bio-protocol (2021-03-05)
M Fernanda Palominos, Andrea Calixto
PMID33659570
ABSTRACT
Quantification of intestinal colonization by pathogenic or commensal bacteria constitute a critical part of the analysis to understand host-microbe interactions during different time points of their interplay. Here we detail a method to isolate non-pathogenic and pathogenic bacteria from C. elegans intestines, and classify gut phenotypes induced by bacterial pathogens using fluorescently-tagged bacteria. Furthermore, these methods can be used to isolate and identify new culturable bacterial species from natural microbiomes of wild nematodes.
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Sigma-Aldrich
Calcium chloride dihydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%
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