Skip to Content
Merck
  • Complement activation and inflammation triggered by model biomaterial surfaces.

Complement activation and inflammation triggered by model biomaterial surfaces.

Journal of biomedical materials research (1998-06-25)
L Tang, L Liu, H B Elwing
ABSTRACT

Biomaterial-mediated complement activation repeatedly has been invoked as a trigger of phagocyte reactions and inflammation. However, a direct correlation between complement activation and inflammatory responses to biomaterial surfaces has yet to be established. Using an animal implantation model and gold surfaces bearing various thiol-linked functionalities, we investigated the potency of different surface groups in prompting complement activation in vitro and surface-mediated accumulation of inflammatory cells in vivo. Among the surfaces tested, mercaptoglycerol- and mercaptoethanol-bearing surfaces engendered the strongest inflammatory responses, as reflected by the accumulation of large numbers of adherent neutrophils and monocytes/macrophages. In contrast, L-cysteine-coated surfaces caused only minor inflammatory responses, and both glutathione-modified and untreated gold implants attracted minimal numbers of inflammatory cells. The accumulation of inflammatory cells on mercaptoglycerol surfaces appears to arise from surface-mediated complement activation because complement-depleted animals failed to exhibit inflammatory responses to mercaptoglycerol-modified implants. Furthermore, there is a close relationship between surface-mediated complement activation (as measured by in vitro iC3b/C5b-9 generation and C3 deposition) and in vivo inflammatory responses. At least in this animal model and with these model surfaces, our results indicate that surface-mediated complement activation can be responsible for the subsequent accumulation of inflammatory cells on implant surfaces.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Thioglycerol, ≥99.0% (GC)
Sigma-Aldrich
1-Thioglycerol, ≥97%
Sigma-Aldrich
1-Thioglycerol, BioXtra, ≥97%
Sigma-Aldrich
1-Thioglycerol, liquid, BioReagent, suitable for cell culture, ≥97% (titration)