Skip to Content
Merck
  • Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control.

Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control.

Nature communications (2015-03-31)
Irene Gallina, Camilla Colding, Peter Henriksen, Petra Beli, Kyosuke Nakamura, Judith Offman, David P Mathiasen, Sonia Silva, Eva Hoffmann, Anja Groth, Chunaram Choudhary, Michael Lisby
ABSTRACT

DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1--together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins--define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated proteins in response to genotoxic stress. The diversity of proteins that localize to INQ indicates that other biological processes such as cell cycle progression, chromatin and mitotic spindle organization may also be regulated through INQ. Similar to Cmr1, its human orthologue WDR76 responds to proteasome inhibition and DNA damage by relocalizing to nuclear foci and physically associating with CCT, suggesting an evolutionarily conserved biological function. We propose that Cmr1/WDR76 plays a role in the recovery from genotoxic stress through regulation of the turnover of sumoylated and phosphorylated proteins.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 L
In Stock
Details...
₩200,361
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride solution, 0.85%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Sign Into View Organizational & Contract Pricing
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
(S)-(+)-Camptothecin, ≥90% (HPLC), powder
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Adenine, BioReagent, suitable for plant cell culture, ≥99%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Adenine, ≥99%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
L-Lysine, ≥98% (TLC)
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, tablet
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, BioXtra, ≥99.5% (AT)
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride solution, 5 M
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Adenine, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
L-Lysine, crystallized, ≥98.0% (NT)
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sign Into View Organizational & Contract Pricing
Adenine, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
Supelco
Sodium chloride, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
Supelco
Adenine, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SAFC
Sodium chloride solution, 5 M
Sign Into View Organizational & Contract Pricing
Supelco
L-Lysine, analytical standard
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing