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Improved HPLC Selectivity of Discovery® BIO Wide Pore RP Phases

Improved HPLC Selectivity of Discovery® BIO Wide Pore RP Phases application for HPLC

Materials

analytical column

Product No.
Description
Pricing

567202-U

Discovery® BIO Wide Pore C18 HPLC Column

3 μm particle size, L × I.D. 15 cm × 2.1 mm

CONDITIONS

column

Discovery BIO Wide Pore C18, 15 cm x 4.6 mm I.D., 5 μm particles vs. competitive wide pore C18 column of same dimensions (568222-U)

mobile phase

[A] 0.1% TFA in 80:20 water:acetonitrile; [B] 0.1% TFA in 66:34 water:acetonitrile

Gradient of 0 to 100% B in 14 min. after 1 min. delay

gradient

0 to 100% B in 14 min. after 1 min. delay

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 220 nm

injection

10 μL

sample

peptide mixture (Sigma P2693), ~0.25 μg each peptide in 0.1% TFA

Description

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS