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Merck
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Key Documents

05-949

Sigma-Aldrich

Anti-Histidine Tagged Antibody, clone HIS.H8

clone HIS.H8, Upstate®, from mouse

동의어(들):

HIS Tag Antibody

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.43

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

클론

HIS.H8, monoclonal

종 반응성(상동성에 의해 예측)

all

제조업체/상표

Upstate®

기술

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG2b

배송 상태

dry ice

타겟 번역 후 변형

unmodified

일반 설명

His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems.

특이성

Recognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag.

면역원

6x His synthetic peptide.

애플리케이션

Immunocytochemistry:
2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells.

Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags

RNA Binding Protein (RBP)
This Anti-Histidine Tagged Antibody, clone HIS.H8 is validated for use in IC, IP, WB for the detection of Histidine Tagged.

품질

Routinely evaluated by western blot and immunocytochemistry on transfected HeLa cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells.

물리적 형태

Format: Purified
Purified
Purified mouse monoclonal IgG2b in buffer containing PBS, pH 7.4 with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
RIPA lysates of transfected HeLa cells.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


시험 성적서(COA)

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