07-013

Anti-MeCP2 Antibody

Upstate^®, from rabbit

• 동의어(들): MeCP-2 protein, mental retardation, X-linked 16, mental retardation, X-linked 79, methyl CpG binding protein 2, methyl CpG binding protein 2 (Rett syndrome)
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: rat, mouse
• 포장: antibody small pack of 25 μg
• 제조업체/상표: Upstate^®
• 기술: western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_004983
• UniProt 수납 번호: P51608
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Mecp2(17257) ; rat ... Mecp2(29386)

설명

일반 설명

NA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

특이성

Predicted to cross-react with human based on the conservation of the immunogenic sequence.

Recognizes MeCP2, Mr 75 kDa. A protein at ~75 kDa was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report.

면역원

KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes.

애플리케이션

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-MeCP2 Antibody (rabbit polyclonal antibody) validated in WB to detect MeCP2 also known as MeCP-2 protein, mental retardation X-linked 16, mental retardation X-linked 79, methyl CpG binding protein 2.

품질

Evaluated by Western Blot on Mouse Brain lysates.

표적 설명

~75 kDa observed

물리적 형태

Format: Purified

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Mouse Brain lysate.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.