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Merck
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Key Documents

07-018-I

Sigma-Aldrich

Anti-phospho-p70 S6 Kinase (Thr389) Antibody

from rabbit, purified by affinity chromatography

동의어(들):

Ribosomal protein S6 kinase, S6K-beta-1, S6K1, p70S6K1, p70 S6K1, p70-S6K1, p70 S6KA

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

human

종 반응성(상동성에 의해 예측)

zebrafish (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)

기술

inhibition assay: suitable (peptide)
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

phosphorylation (pThr389)

유전자 정보

human ... RPS6KB1(6198)

일반 설명

The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.

특이성

Other homologies: ribosomal protein S6 kinase beta-2 (91% sequence homology).
This antibody recognizes the AGC-kinase C-terminal of Ribosomal protein S6 kinase beta-1 phosphorylated at Thr389.

면역원

Epitope: AGC-kinase C-terminal
KLH-conjugated linear peptide corresponding to the AGC-kinase C-terminal of human Ribosomal protein S6 kinase beta-1
phosphorylated at Thr389.

애플리케이션

Detect phospho-p70 S6 Kinase (Thr389) using this Anti-phospho-p70 S6 Kinase (Thr389) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

품질

Evaluated by Western Blot (Peptide Inhibition) Analysis in MCF7 cell lysate and MCF7 cell lysate with non-phospho or phospho peptide.

Western Blot (Peptide Inhibition) Analysis: 1.0 µg/mL of this antibody detected p70 S6 Kinase phosphorylated at Thr389 in 10 µg of MCF7 cell lysate and MCF7 cell lysate incubated with a non-phospho peptide.

표적 설명

~70 kDa observed

물리적 형태

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
MCF7 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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Females from many mosquito species feed on blood to acquire nutrients for egg development. The oogenetic cycle has been characterized in the arboviral vector Aedes aegypti, where after a bloodmeal, the lipid transporter lipophorin (Lp) shuttles lipids from the midgut
Samer Wassim El-Kadi et al.
Journal of applied physiology (Bethesda, Md. : 1985) (2018-08-03)
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Nathan M Belliveau et al.
Nature communications, 14(1), 5770-5770 (2023-09-19)
Neutrophils are the most abundant leukocyte in humans and provide a critical early line of defense as part of our innate immune system. We perform a comprehensive, genome-wide assessment of the molecular factors critical to proliferation, differentiation, and cell migration
A De Leo et al.
Cell death & disease, 6, e1876-e1876 (2015-09-04)
Autophagy, an important degradation system involved in maintaining cellular homeostasis, serves also to eliminate pathogens and process their fragments for presentation to the immune system. Several viruses have been shown to interact with the host autophagic machinery to suppress or
Ying Chen et al.
Frontiers in physiology, 8, 482-482 (2017-07-27)
Low-birth-weight (LBWT) neonates experience restricted muscle growth in their perinatal life. Our aim was to investigate the mechanisms that contribute to slower skeletal muscle growth of LBWT neonatal pigs. Twenty-four 1-day old male LBWT (816 ± 55 g) and normal-birth-weight

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