219343

Cathepsin S, Human, Recombinant, E. coli

Cathepsin S, Human, Recombinant, E. coli, is prepared without a tag or fusion protein. A major lysosomal cysteine proteinase with high specific activity.

• 동의어(들): Cathepsin S Protein
• 효소 위원회 번호: 3.4.22.27 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Quality Level: 100
• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥90% (SDS-PAGE)
• 형태: liquid
• 특이 활성도: ≥30,000 mU/mg protein
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; avoid repeated freeze/thaw cycles
• 기술: cell based assay: suitable
• 단백질 ID 수납 번호: AAC37592; AAH02642
• UniProt 수납 번호: P25774
• 배송 상태: wet ice
• 저장 온도: −70°C
• 유전자 정보: human ... CTSS(1520)

설명

일반 설명

Note: 1 mU = 1 milliunit.

Research area: Cell Signaling

Recombinant, human cathepsin S expressed in E. coli without a tag or fusion protein. A major lysosomal cysteine proteinase that is preferentially expressed in macrophages and microglia. Shown to be involved in the processing of antigenic peptides for presentation by MHC Class II molecules on the surface of antigen-presenting cells by mediating invariant (Ii) chain degradation. Inhibition of cathepsin S results in impaired antigen-presentation.

애플리케이션

Cathepsin S, Human, Recombinant, E. coli has been used in cathepsin digestion reactions.

생화학적/생리학적 작용

Cathepsin S is a cysteine protease that plays a role in degradation of class II complexes in human B lymphocytes. It possesses endoproteolytic activity and aids in processing and presenting antigens to immune cells, thereby influencing the immune response. Cathepsin S is involved in facilitating the degradation of damaged or unwanted proteins within the endo-lysosomal pathway. Dysregulation of Cathepsin S activity leads to the development of various diseases such as arthritis, cancer, and cardiovascular diseases.

경고

Toxicity: Harmful (C)

단위 정의

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-VVR-AMC per min at 37°C, pH 6.5. Note: 1 mU = 1 milliunit.

물리적 형태

In 35 mM potassium phosphate, 35 mM Sodium acetate, 2 mM DTT, 2 mM EDTA, 50% ethylene glycol, pH 6.5.

재구성

Following initial thaw, aliquot and freeze (-70°C).

기타 정보

Liuzzo, J.P., et al. 1999. Mol. Med.5, 334.
Riese, R.J., et al. 1998. J. Clin. Invest.101, 2351.
Sukhova, G.K., et al. 1998. J. Clin. Invest.102, 576.
Kirschke, H., and Wiederanders, B. 1994. Methods Enzymol.244, 500.
Xin, X.Q., et al. 1992. Arch. Biochem. Biophys.299, 334.
Kirschke, H., et al. 1989. Biochem. J.264, 467.

법적 정보

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302,H373
• 예방조치 성명서: P260 - P264 - P270 - P301 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Oral - STOT RE 2 Oral
• 표적 기관: Kidney
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1