추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, rat, sheep, guinea pig, pig
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... ADORA1(134)
특이성
Recognizes Adenosine A1 receptor (A1R). Recognizes the 79 kDa and 39 kDa bands of in cortical membranes from rat, porcine and lamb. The 79 kDa band can be converted to 39 kDa band by agonist or antagonist treatment and could represent a A1R dimers.
면역원
Amino acid sequence corresponding to the 3rd extracellular domain (amino acids 163-176, GEPVIKCEFEKVIS) of the rat A1R gene.
애플리케이션
Anti-Adenosine A1 Receptor Antibody detects level of Adenosine A1 Receptor & has been published & validated for use in ELISA, IC, IH & WB.
Immunohistochemistry: 10-50 μg/mL on frozen tissue sections. Light fixation is recommended. 2% 0.2% picric acid {Cooke, HJ (1999) AJP Gastrointest Liver Physiol 276:4516-4626}
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)
Optimal working dilutions must be determined by end user.
Immunocytochemistry: Caufield, 2003. Light 2% PFA fixation 5-10 minutes --long fixations lower staining intensity.
Western blot: 1-10 μg/mL (Chemiluminescence technique): Membrane preparations are recommended for enhanced signals {Smith et al (2001) Biochem Pharmacology 61:237-244} Higher concentrations of the antibody may be necessary if calorimetric detection or milk-based antibody diluent are used.
ELISA: 1:10,000-1:50,000 (50-100 ng A1R peptide (Cat. Number AG289)/well)
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
Neurotransmitters & Receptors
Circadian Rhythm & Sleep
표적 설명
79 kDa and 39 kDa
물리적 형태
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.
ImmunoAffinity Purified
저장 및 안정성
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Brain tissue
Brain tissue
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Cátia Vieira et al.
Mediators of inflammation, 2014, 254640-254640 (2014-09-12)
Purinergic signalling is remarkably plastic during gastrointestinal inflammation. Thus, selective drugs targeting the "purinome" may be helpful for inflammatory gastrointestinal diseases. The myenteric neuromuscular transmission of healthy individuals is fine-tuned and controlled by adenosine acting on A(2A) excitatory receptors. Here
Patrycja Bolewska et al.
Neuroscience journal, 2019, 5950818-5950818 (2019-07-06)
There is an excellent correlation between the age when alcohol consumption begins and the likelihood of lifelong problems with alcohol abuse. Alcohol use often begins in adolescence, a time marked by brain development and maturation of numerous brain systems. Rats
Carina Herman-de-Sousa et al.
Cells, 9(3) (2020-03-12)
Human subcutaneous fibroblasts (HSCF) challenged with inflammatory mediators release huge amounts of ATP, which rapidly generates adenosine. Given the nucleoside's putative relevance in wound healing, dermal fibrosis, and myofascial pain, we investigated the role of its precursor, AMP, and of
Ella E Bauer et al.
Neural plasticity, 2020, 5859098-5859098 (2020-05-14)
Adenosine acts as a key regulator of striatum activity, in part, through the antagonistic modulation of dopamine activity. Exercise can increase adenosine activity in the brain, which may impair dopaminergic functions in the striatum. Therefore, long-term repeated bouts of exercise
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