MABS82

Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1

clone 10G7.1, from mouse

• 동의어(들): Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, Short name=P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, Serine/threonine-protein kinase 14A, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alp
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 10G7.1, monoclonal
• 종 반응성: human, mouse
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NP_003152
• UniProt 수납 번호: P23443
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pThr389)
• 유전자 정보: human ... RPS6KB1(6198)

설명

일반 설명

The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.

특이성

This antibody recognizes p70 S6 Kinase phosphorylated at (Thr389). This antibody also recognizes isoforms Alpha I and Alpha II.

면역원

Epitope: Phosphorylated (Thr389)

KLH-conjugated linear peptide corresponding to mouse p70 S6 Kinase phosphorylated at (Thr389).

애플리케이션

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p70 S6 Kinase in A431 cells.

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

This Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1 is validated for use in Western Blotting, ICC for the detection of phospho-p70 S6 Kinase (Thr389).

품질

Evaluated by Western Blot in untreated and serum treated NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected p70 S6 Kinase on 10 µg of untreated and serum treated NIH/3T3 cell lysates.

표적 설명

~70/85 kDa observed. This protein recognizes isoforms Alpha I and Alpha II. Uncharacterized bands appear at ~18 and 200 kDa in some lysates depending on treatment conditions.

결합

Replaces: 04-392

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Untreated and serum treated NIH/3T3 cell lysates

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable