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Merck
모든 사진(1)

문서

10107921001

Roche

Nuclease S7

Micrococcal nuclease, from Staphylococcus aureus

동의어(들):

s7 nuclease

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모든 사진(1)

About This Item

UNSPSC 코드:
12352204

생물학적 소스

Staphylococcus aureus

Quality Level

100

형태

lyophilized

특이 활성도

~15,000 U/mg

포장

pkg of 15,000 U

제조업체/상표

Roche

최적 pH

7.5

배송 상태

wet ice

관련 카테고리

애플리케이션

  • Removal of endogenous RNA from in vitro translation systems
  • RNA sequencing experiments
Nuclease S7 has been used in ribonuclease selection process for ribosome profiling.

생화학적/생리학적 작용

Nuclease S7 is an endonuclease that mainly cleaves single-stranded substrates. It also cleaves double-stranded DNA or RNA. It cleaves the 5′-phosphodiester bonds of RNA and DNA to yield 3′-mononucleotides and oligonucleotides. Activity of the enzyme is dependent on Ca2+ and can be completely inactivated by the addition of EDTA. Nuclease S7 cleavage is highly specific to AT rich region than GC on the DNA.

단위 정의

One unit releases a sufficient amount of acid-soluble oligonucleotides to produce an increase of 1.0 at A260.

저장 및 안정성

Store unopened reagent at 2-8 °C; when diluted to 1 mg/mL, store at -15 to -25 °C.

기타 정보

For life science research only. Not for use in diagnostic procedures.

픽토그램

Exclamation markHealth hazard
GHS07,GHS08

신호어

Danger

유해 및 위험 성명서

H315,H317,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)
Ariane Lismer et al.
Nucleic acids research, 48(20), 11380-11393 (2020-10-18)
Advancing the molecular knowledge surrounding fertility and inheritance has become critical given the halving of sperm counts in the last 40 years, and the rise in complex disease which cannot be explained by genetics alone. The connection between both these
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
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