IPTG-RO

Isopropyl-β-D-thiogalactoside

mol wt 238.3, pkg of 1 g (10724815001), pkg of 5 g (11411446001)

• 동의어(들): Isopropyl β-D-1-thiogalactopyranoside, iptg, IPTG, Isopropyl β-D-thiogalactoside
• 실험식(Hill 표기법): C₉H₁₈O₅S
• CAS Number: 367-93-1
• Molecular Weight: 238.30
• Beilstein: 4631
• MDL number: MFCD00063273
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329815691

속성

• 형태: powder
• 분자량: 238.3
• 포장: pkg of 1 g (10724815001); pkg of 5 g (11411446001)
• 제조업체/상표: Roche
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• SMILES string: CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

설명

일반 설명

Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

애플리케이션

Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

기타 정보

Non-metabolizable galactose analog.

For life science research only. Not for use in diagnostic procedures.

The amount of labeled DNA depends on:

• amount of template DNA
• purity of template DNA
• conformation of template DNA
• average size of labeled fragment

규격

Assay Time: 50minutes
Specific Activity: The described standard assay will results in a specific activity of 1.8 x 10⁹ dpm/μg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.

제조 메모

Sample Material
10 ng to 3μg template DNA, linearized and at least 100 to 200bp long

Working concentration: Final concentration should be 0.2 mM.
Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable