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Merck
모든 사진(1)

주요 문서

83850

Sigma-Aldrich

Ribonucleic acid from Torula utilis

Mr 5000-8000

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About This Item

MDL number:
UNSPSC 코드:
12352106
NACRES:
NA.51

Grade

for molecular biology

Quality Level

양식

solid

분자량

Mr 5000-8000

불순물

≤10% water

solubility

0.1 M acetate buffer pH 5.0: soluble 10 mg/mL

배송 상태

wet ice

저장 온도

−20°C

일반 설명

RNA from yeast is used in molecular biology laboratories as a carrier during nucleic acid purifications. When used at 10-20 μg/ml, the tRNA acts as a potent co-precipitant that allows for maximal RNA recovery (when working with limited samples).

애플리케이션

Ribonucleic acid from Torula utilis was used as a standard in ribonuclease activity assay of certain ribonuclease family members. It was also used as a standard to determine RNA concentration by orcinol assay.
Suitable for use in nucleic acid purifications.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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Russel J Lander et al.
Biotechnology and bioengineering, 79(7), 776-784 (2002-09-05)
Preparative-scale purification of plasmid DNA has been attempted by diverse methods, including precipitation with solvents, salts, and detergents and chromatography with ion-exchange, reversed-phase, and size-exclusion columns. Chromatographic methods such as hydrophobic interaction chromatography (HIC), reversed phase chromatography (RPC), and size
Debi Ranjan Tripathy et al.
Analytical biochemistry, 437(2), 126-129 (2013-03-19)
The ribonuclease (RNase) activity of ribonucleases has been assayed by observing the change in fluorescence intensity of ethidium bromide on binding with yeast RNA. The binding of EtBr with RNA was monitored via UV-vis and fluorimetric methods. The degradation of

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