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Key Documents

B0390

Sigma-Aldrich

Bilirubin Oxidase from Myrothecium verrucaria

greener alternative

lyophilized powder, 15-65 units/mg protein

동의어(들):

MvBOx, Bilirubin:oxygen oxidoreductase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

fungus (Myrothecium verrucaria)

형태

lyophilized powder

특이 활성도

15-65 units/mg protein

구성

Protein, ≥15% biuret

저장 조건

(Keep container tightly closed in a dry and well-ventilated place.)

환경친화적 대안 제품 특성

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

기술

toxicology assay: suitable

환경친화적 대안 카테고리

배송 상태

dry ice

저장 온도

−20°C

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관련 카테고리

일반 설명

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.

Bilirubin oxidase (BOD) belongs to the subclass of the multicopper oxidase family. It is usually found in fungi.Bilirubin Oxidase is composed of three cupredoxin-like domains that result in two active sites formed by four copper ions. Research area: Apoptosis

애플리케이션

Bilirubin Oxidase from Myrothecium verrucaria has been used to eliminate the interference of bilirubin. It has also been used to chemically modify with fluorescein derivative (FS) and combined with multivariate calibration to analyse direct bilirubin (dBR) and total bilirubin (tBR).
Bilirubin oxidase is used to degrade bilirubin. Bilirubin oxidase, from Myrothecium verrucaria, may be used to determine free hemoglobin in icteric specimens . It also has potential application in dye effluent decolorization and is a potential treatment for neonatal jaundice.

생화학적/생리학적 작용

Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. Oxygen is the electron acceptor and is reduced to water. The enzyme is used for diagnostic analysis of bilirubin in serum during medical examinations of the serum. It has also been used as an enzymatic catalyst for the cathode of biofuel cells that work under neutral conditions. It is a monomeric protein with a molecular mass of 60 kDa and high reactivity at neutral pH. BOD participates in porphyrin and chlorophyll metabolism.
Bilirubin oxidase (BOD) is capable of oxidizing laccase substrates.

단위 정의

One unit will oxidize 1.0 μmole of bilirubin per min at pH 8.4 at 37 °C.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

Luciano Dos Santos et al.
Physical chemistry chemical physics : PCCP, 12(42), 13962-13974 (2010-09-21)
The 'blue copper' enzyme bilirubin oxidase from Myrothecium verrucaria shows significantly enhanced adsorption on a pyrolytic graphite 'edge' (PGE) electrode that has been covalently modified with naphthyl-2-carboxylate functionalities by diazonium coupling. Modified electrodes coated with bilirubin oxidase show electrocatalytic voltammograms
Kimihiko Mizutani et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 66(Pt 7), 765-770 (2010-07-08)
Bilirubin oxidase (BOD), a multicopper oxidase found in Myrothecium verrucaria, catalyzes the oxidation of bilirubin to biliverdin. Oxygen is the electron acceptor and is reduced to water. BOD is used for diagnostic analysis of bilirubin in serum and has attracted
A'edah Abu-Bakar et al.
Toxicology and applied pharmacology, 257(1), 14-22 (2011-08-30)
We have previously shown that bilirubin (BR), a breakdown product of haem, is a strong inhibitor and a high affinity substrate of the mouse cytochrome P450 2A5 (CYP2A5). The antioxidant BR, which is cytotoxic at high concentrations, is potentially useful
Laith Hussein et al.
Physical chemistry chemical physics : PCCP, 13(13), 5831-5839 (2011-02-18)
The fabrication process of buckypapers (BPs) made from stable suspensions of as-received or functionalized multi-walled carbon nanotubes (MWCNTs) with high purity (97.5 wt%, Baytubes), their characterization and their utilization towards novel biofuel cell electrode applications are reported. The BPs can
Emmanuel Suraniti et al.
Analytical chemistry, 83(7), 2824-2828 (2011-03-17)
Immobilization and electrical wiring of enzymes is of particular importance for the elaboration of efficient biosensors and can be cumbersome. Here, we report a fast and easy protocol for enzyme immobilization, and as a proof of concept, we applied it

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