추천 제품
생물학적 소스
mouse
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
BN-34, monoclonal
포함
15 mM sodium azide
기술
capture ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: 1:4,000
동형
IgG1
응용 분야
research pathology
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
Biotin is a water-soluble vitamin. It is also called as vitamin B8. Biotin is a bicyclic compound that has a uredo ring and a tetrahydrothiophene ring.
Monoclonal Anti-Biotin recognizes the free biotin molecule and biotin conjugated to various immunoglobulins in ELISA and immunohistochemical techniques. Specificity was verified by using biotinylated goat antibodies reactive against human and rabbit antigens coated on microwell plates.
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.
면역원
Biotin-KLH conjugate
Biotinylated keyhole limpet hemocyanin (KLH)
애플리케이션
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Monoclonal Anti-Biotin antibody has been used in proximity ligation assay (PLA), cell surface biotinylation assay and western blotting.
The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
Utilized in a variety of applications including blotting, immunocytochemistyr, insitu nucleic acid hybridization, ELISA, fluorescent activated cell-sorting (FACS) and electron microscopy.Titer is approximately 1:2000 by ELISA but should be optimized by the individual user for their application of choiceAn essential vitamin, biotin is required by living organisms and cells in culture. Avidin′s high affinity for biotin is often exploited in immuno-based assays. While standard assay methods using the avidin-biotin enzyme complex will suffice for most studies, there are occasions when enhanced sensitivity is needed to detect smaller amounts of antigen or localize low density antigens in histologic sections. Conventional immunoassay methods are improved by the use of Monoclonal Anti-Biotin which enhances the sensitivity of avidin-biotin immunoassays by bridging a second layer of avidin-biotin-enzyme complex. This antibody can be used in many other applications where biotin can be introduced as a target label.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.
생화학적/생리학적 작용
Biotin may participate in the replication and transcription of DNA. It acts as a coenzyme in the fatty acid biosynthesis, glyconeogenesis, and catabolism of several branched-chain amino acids and odd-chain fatty acids.
제조 메모
Developed in BALB/c mice immunized with biotinylated KLH. Mouse myeloma cells and splenocytes are fused together to form a BN-34 hybridoma.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Erik C Yusko et al.
Nature nanotechnology, 6(4), 253-260 (2011-02-22)
Synthetic nanopores have been used to study individual biomolecules in high throughput, but their performance as sensors does not match that of biological ion channels. Challenges include control of nanopore diameters and surface chemistry, modification of the translocation times of
Israr-ul H Ansari et al.
The Journal of biological chemistry, 290(38), 23110-23123 (2015-08-05)
The negative charge of phosphatidylserine in lipid bilayers of secretory vesicles and plasma membranes couples the domains of positively charged amino acids of secretory vesicle SNARE proteins with similar domains of plasma membrane SNARE proteins enhancing fusion of the two
Direct visualization of newly synthesized target proteins in situ.
Susanne TD, et al.
Nature Methods, 12(5), 411-411 (2015)
Characterization of P4 ATPase phospholipid translocases (flippases) in human and rat pancreatic beta cells: their gene silencing inhibits insulin secretion.
Israr-ul HA, et al.
The Journal of Biological Chemistry, M115-M115 (2015)
J A Woo et al.
Cell death and differentiation, 22(6), 921-934 (2015-02-24)
The accumulation of amyloid-β protein (Aβ) is an early event associated with synaptic and mitochondrial damage in Alzheimer's disease (AD). Recent studies have implicated the filamentous actin (F-actin) severing protein, Cofilin, in synaptic remodeling, mitochondrial dysfunction, and AD pathogenesis. However
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