추천 제품
생물학적 소스
rabbit
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
buffered aqueous solution
분자량
antigen ~89 kDa
종 반응성
human
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
농도
~1.5 mg/mL
기술
indirect immunofluorescence: 10-20 μg/mL using HeLa cells
western blot: 1-2 μg/mL using HEK-293T cells expressing human Cullin-4A
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... CUL4A(8451)
일반 설명
Cullin-4 (Cul4) proteins are the core components of ubiquitin E3 ligases. Mammalian cells express two distinct but highly homologous cullin-4 proteins, cullin-4A and cullin-4B.
Cullin-4A is a protein encoded by CUL4A gene in humans.
애플리케이션
Anti-Cullin-4A antibody produced in rabbit is used for Immunofluorescence at a concentration of 10-20mg/mL using HeLa cells and is also used for western blotting at a concentration of 1-2μg/mL using HEK-293T cells expressing human Cullin-4A.
생화학적/생리학적 작용
Cullin-4 (Cul4) proteins are components of ubiquitin E3 ligases that regulate cell cycle, genome stability, DNA replication and transcription. CUL4-based E3 ligase plays an important role for the repression of photomorphogenesis in plants (like Arabidopsis thaliana).
Cullin-4A (Cul4A) is involved in identifying specific targets for ubiquitylation. The Cul4A gene is known to be amplified and up-regulated in breast carcinoma and many other tumor types. Cul4A associates with damage-specific DNA binding proteins (DDB1 and DDB2). It functions as substrate recruiting factors for Cul4A-Roc1 ligases. Cul4A is known to ubiquitylate several target proteins including DDB1/DDB2, CDT1 (chromatin licensing and dna replication factor 1), DNA repair protein complementing XP-C cells (XPC), histone H2A, H3 and H4. In response to DNA damage Cul4A complex regulates protein stability of p53 and mouse double minute 2 homolog (MDM2)/ human double minute 2 homolog (HDM2).
물리적 형태
solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
DNA damage binding protein component DDB1 participates in nucleotide excision repair through DDB2 DNA-binding and cullin 4A ubiquitin ligase activity
Li J, et al.
Cancer Research, 66(17), 8590-8597 (2006)
The human homologue for the Caenorhabditis elegans cul-4 gene is amplified and overexpressed in primary breast cancers
Chen LC, et al.
Cancer Research, 58(16), 3677-3683 (1998)
Damon Banks et al.
Cell cycle (Georgetown, Tex.), 5(15), 1719-1729 (2006-07-25)
The CUL4-ROC1 E3 ligase complex regulates genome stability, replication and cell cycle progression. A novel WD40 domain-containing protein, L2DTL/CDT2 and PCNA were identified as proteins associated with CUL4/DDB1 complexes. Inactivation of CUL4A, L2DTL, PCNA, DDB1 or ROC1 induced p53 stabilization
Leigh Ann Higa et al.
Cell cycle (Georgetown, Tex.), 5(1), 71-77 (2005-12-03)
The CUL4 (cullin 4) proteins are the core components of a new class of ubiquitin E3 ligases that regulate replication and transcription. To examine the roles of CUL4 in cell cycle regulation, we analyzed the effect of inactivation of CUL4
Haodong Chen et al.
The Plant cell, 18(8), 1991-2004 (2006-07-18)
Repression of photomorphogenesis in Arabidopsis thaliana requires activity of the COP9 signalosome (CSN), CDD, and COP1 complexes, but how these three complexes work in concert to accomplish this important developmental switch has remained unknown. Here, we demonstrate that Arabidopsis CULLIN4
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