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Merck
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Key Documents

H7541

Sigma-Aldrich

IL-10 human

HumanKine®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

동의어(들):

IL-10

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About This Item

MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.77

product name

Interleukin-10 human, IL-10, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

생물학적 소스

human

Quality Level

재조합

expressed in HEK 293 cells

분석

≥95% (SDS-PAGE)

형태

lyophilized powder

효능

≤1.5 ng/mL EC50

품질

endotoxin tested

분자량

dimer 17 kDa (glycosylated)

기술

cell culture | mammalian: suitable

불순물

≤1EU/mg

UniProt 수납 번호

저장 온도

−20°C

유전자 정보

human ... IL10(3586)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Interleukin-10 gene with five exons is mapped to human chromosome 1. The gene codes for 35 kD protein containing 178 amino acids. IL-10 is a homodimer with two, non-covalently bonded monomers.

애플리케이션

Interleukin-10 human has been used to study its effect on activation of endogenous signal transducer and activator of transcription 1 (STAT1)/STAT3 in the knock-in, in A549 human lung adenocarcinoma cell line.

생화학적/생리학적 작용

IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

물리적 형태

Lyophilized from a 0.2 μm filtered solution of 10mM Tris-HCl pH 7.4 + 150mM NaCl

제조 메모

HumanKine Interleukin-10 (IL-10) is expressed in human HEK 293 cells as a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

분석 메모

The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse master cell line) in presence of 200 pg/mL IL-4.

법적 정보

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

이미 열람한 고객

Biology of interleukin-10
Sabat R, et al.
Cytokine & Growth Factor Reviews, 21(5), 331-344 (2010)
Tagging of genomic STAT3 and STAT1 with fluorescent proteins and insertion of a luciferase reporter in the cyclin D1 gene provides a modified A549 cell line to screen for selective STAT3 inhibitors
Samsonov A, et al.
PLoS ONE, 8(7) (2013)
Robert Sabat et al.
Cytokine & growth factor reviews, 21(5), 331-344 (2010-12-01)
Interleukin (IL)-10 is the most important cytokine with anti-inflammatory properties besides TGF-β and IL-35. It is produced by activated immune cells, in particular monocytes/macrophages and T cell subsets including Tr1, Treg, and Th1 cells. IL-10 acts through a transmembrane receptor
Da-En Cheng et al.
Molecular medicine reports, 10(5), 2389-2394 (2014-09-19)
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Piyatida Tangteerawatana et al.
The Southeast Asian journal of tropical medicine and public health, 45(3), 517-530 (2014-07-01)
Immunity to malaria can be acquired but only after repeat exposures to polymorphic Plasmodium. However, the development of clinical outcomes during P. falciparum infection is not clearly understood. This study elucidated whether monocytes, neutrophils and pro/anti-inflammatory cytokines were associated with

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