M7927

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD01633615
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen, ERK-1 44 kDa; antigen, ERK-2 42 kDa
• 종 반응성: mouse, rat, human
• 기술: immunoprecipitation (IP): 5 μg using NIH 3T3 mouse fibroblast lysates; microarray: suitable; western blot: 1:5,000 using NIH 3T3 mouse fibroblast lysate or rat brain extract
• UniProt 수납 번호: P27361
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MAPK3(5595) ; mouse ... Mapk3(26417) ; rat ... Mapk3(50689)

설명

일반 설명

Mitogen-activated protein kinase 3 (ERK1) is mapped to human chromosome 16p11.2. It has a serine/threonine domain and a transactivation domain (TAD) and a nuclear localization sequence (NLS).

특이성

By immunoblotting, the antibody reacts with ERK-1 and ERK-2.

면역원

synthetic peptide corresponding to amino acids 351-368 of human ERK-1.

애플리케이션

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit has been used in:

• immunofluorescence
• immunoprecipitation
• western blotting.

생화학적/생리학적 작용

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), c-Jun N-terminal kinases (JNK) and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.

물리적 형태

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)