P7626

Phenylmethanesulfonyl fluoride

powder, ≥98.5% (GC)

• 동의어(들): α-Toluenesulfonyl fluoride, Benzylsulfonyl fluoride, PMSF, Phenylmethylsulfonyl fluoride
• 실험식(Hill 표기법): C₇H₇FO₂S
• CAS Number: 329-98-6
• Molecular Weight: 174.19
• Beilstein: 2088311
• EC Number: 206-350-2
• MDL number: MFCD00007424
• UNSPSC 코드: 12352202
• PubChem Substance ID: 24898863
• NACRES: NA.77

속성

• 제품명: Phenylmethanesulfonyl fluoride, ≥98.5% (GC)
• 생물학적 소스: synthetic
• 분석: ≥98.5% (GC)
• 양식: powder
• mp: 91-94 °C
• solubility: dry solvents (ethanol, methanol, and 2-propanol): 200 mM (Stock solution are stable for months at 4°C.); H₂O: unstable
• SMILES string: FS(=O)(=O)Cc1ccccc1
• InChI: 1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
• InChI key: YBYRMVIVWMBXKQ-UHFFFAOYSA-N

설명

일반 설명

Phenylmethanesulfonyl fluoride (PMSF) is a widely used serine protease inhibitor, effective against such enzymes as chymotrypsin, thrombin, and trypsin. PMSF acts as an inhibitor via sulfonation of the hydroxyl residues of serine residues at the reactive sites of serine proteases.

애플리케이션

PMSF is often used in lysis buffer to assist in preserving proteins of interest during protein isolation and sample preparation, by inhibiting proteases that would otherwise degrade proteins after cell or tissue lysis. PMSF is unstable in aqueous media. Thus stock solutions of PMSF are generally prepared in anhydrous organic solvents (e.g. 100% ethanol, or anhydrous isopropanol) prior to use in aqueous media.

Noted general features and benefits of PMSF include the following:

• Inhibits serine proteases such as trypsin and chymotrypsin
• Also inhibits cysteine proteases (reversible by reduced thiols) and mammalian acetylcholinesterase
• Not as effective or as toxic as DFP
• Effective concentration 0.1-1 mM
• Half-life = 1 hr. at pH 7.5

Phenylmethanesulfonyl fluoride has been used in following applications:

• cell fractionation.
• used as a supplement in nuclear protein extraction.
• inhibitor of cholesterol esterase (CE) and pseudocholinesterase (PCE).
• used for the collection of blood prior to centrifugation to quantify plasma ANP levels.

생화학적/생리학적 작용

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• 픽토그램: GHS06,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H301,H314
• 예방조치 성명서: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Acute Tox. 3 Oral - Skin Corr. 1B
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges