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Merck
모든 사진(3)

Key Documents

P8811

Sigma-Aldrich

Protease from Streptomyces griseus

powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid

동의어(들):

Actinase E, Pronase E

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352207
eCl@ss:
32160410
NACRES:
NA.75

제품 라인

BioReagent

형태

powder

특이 활성도

≥3.5 units/mg solid

기술

cell culture | embryo: suitable

불순물

starch, essentially free

저장 온도

−20°C

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특이성

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

애플리케이션

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

기타 정보

An unusually non-specific protease.

품질

Contains approximately 25% calcium acetate.

물리적 특성

Completely inactivated by heating above 80 °C for 15-20 minutes.

단위 정의

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

제조 메모

Collected from culture broth of S. griseus.

픽토그램

Health hazardExclamation mark

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Shantha K Mahadevaiah et al.
Nature, 586(7830), 612-617 (2020-08-21)
Single-cell RNA sequencing of embryos can resolve the transcriptional landscape of development at unprecedented resolution. To date, single-cell RNA-sequencing studies of mammalian embryos have focused exclusively on eutherian species. Analysis of mammalian outgroups has the potential to identify deeply conserved
S Blumberg et al.
European journal of biochemistry, 136(1), 151-154 (1983-10-17)
A series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the N-hydroxysuccinimide ester of S-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with NaOH in aqueous methanol and acidification with HCl. Thus
J Erik Mylroie et al.
Environmental toxicology and chemistry, 40(3), 780-791 (2020-10-13)
Perfluorooctanesulfonic acid (PFOS) is a perfluorinated compound used in many industrial and consumer products. It has been linked to a broad range of adverse effects in several species, including zebrafish (Danio rerio). The zebrafish embryo is a widely used vertebrate
Yan Jiang et al.
Human gene therapy, 30(2), 179-196 (2018-07-20)
Hepatic stellate cells (HSCs) are the primary cell type responsible for liver fibrogenesis. Transforming growth factor beta 1 (TGF-β1) and platelet-derived growth factor (PDGF) are key profibrotic cytokines that regulate HSC activation and proliferation with functional convergence. Dual RNA interference
Xuan Mu et al.
Macromolecular bioscience, 20(1), e1900191-e1900191 (2019-08-23)
Hierarchical molecular assembly is a fundamental strategy for manufacturing protein structures in nature. However, to translate this natural strategy into advanced digital manufacturing like three-dimensional (3D) printing remains a technical challenge. This work presents a 3D printing technique with silk

문서

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

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