SAB4200483

Anti-JAK2 antibody, Mouse monoclonal

clone 10.1.5, purified from hybridoma cell culture

• 동의어(들): Anti-JAK-2, Anti-JTK10, Anti-Janus kinase 2, Monoclonal Anti-JAK2 antibody produced in mouse
• UNSPSC 코드: 12352203
• NACRES: NA.44

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: 10.1.5, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~125 kDa
• 종 반응성: bovine, human
• 농도: ~1.0 mg/mL
• 기술: indirect immunofluorescence: 2.5-5.0 μg/mL using MCF7 cells.; western blot: 2.0-4.0 μg/mL using TF1 total cell extracts.
• UniProt 수납 번호: O60674
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... JAK2(3717)

설명

일반 설명

Monoclonal Anti-JAK2 (mouse IgG1 isotype) is derived from the hybridoma 10.1.5 produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. The Janus kinases (Jak) family of proteins includes four members: Jak1, Jak2, Jak3 and the tyrosine kinase 2 (Tyk2). Jak2 is ubiquitously expressed. It is also expressed in normal and neoplastic mammary epithelial cells.

면역원

synthetic peptide corresponding to an internal sequence of human JAK2 (GeneID 3717), conjugated to KLH.

애플리케이션

Monoclonal Anti-JAK2 antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• flow cytometry

생화학적/생리학적 작용

Janus kinases engage in interactions with the intracellular tails of several cytokine receptors, which result in the activation of members of the signal transducer and activator of transcription (STAT) family of transcription factors. The latter bind specific cognate sequences at the promoter region of genes that regulate proliferation and differentiation. JAK activation plays an important role in cell proliferation, differentiation, migration and apoptosis. A member of this family, JAK2, has been found to induce signaling regulating gene expression of various pro-survival and anti-apoptotic molecules. Furthermore, inhibition of JAK2 signaling triggered cell death in human cancer cells. These findings indicate JAK2 inhibition as a potential therapeutic approach for various cancers.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable