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Merck
모든 사진(1)

Key Documents

SAB4200805

Sigma-Aldrich

Anti-β-Galactosidase antibody, Mouse monoclonal

clone GAL-13, purified from hybridoma cell culture

동의어(들):

Anti-Beta-gal, Anti-Beta-galactosidase, Anti-Lactase

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

항체 형태

purified from hybridoma cell culture

항체 생산 유형

primary antibodies

클론

GAL-13, monoclonal

설명

Research area: Microbiome

형태

buffered aqueous solution

종 반응성

E. coli

포장

antibody small pack of 25 μL

농도

~1.0 mg/mL

기술

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: 0.5-1 μg/mL using anti-Mouse antibody for coating, primary Anti-Beta-GAL antibody followed by addition of β-d-galactosidase enzyme from E. coli and 2-Nitrophenyl β-D-galactopyranoside as substrate.

동형

IgG1

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

Escherichia coli K12 ... Lacz(945006)

일반 설명

Anti-nitric oxide synthase, inducible antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH
Beta galactosidases have been obtained from microorganisms such as fungi, bacteria and yeasts; plants, animals cells and from recombinant sources. This enzyme has two main applications; the removal of lactose from milk products for lactose intolerant people and the production of galactosylated products.Monoclonal Anti-Beta-Galactosidase recognizes soluble Beta-d-galactosidase and retains the enzymatic activity. The antibody reacts with the native enzyme and does not recognize denatured or reduced Beta-galactosidase. The Anti-Beta-Galactosidase antibody maybe useful tool for immunoenzymatic staining of blood and bone marrow smears or tissue sections. In addition, this product may be used as a primary antibody for the detection and purification of recombinant fusion hybrid proteins, which contain Beta-galactosidase (lacZ) in the cloning vector.

면역원

Beta-D-Galactosidase purified from E. coli

애플리케이션

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal has been used in: immunoblotting immunofluorescence fluorescence-activated cell sorting 3 (FACS3) immunohistochemistry
The antibody is recommended to use in various immunological techniques, including ELISA, Immunofluorescence and immunohistology.

생화학적/생리학적 작용

NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, antimicrobial and anti-tumor activities. It is implicated as a pathogenic mediator in a variety of conditions, including central nervous system (CNS) disease states, such as the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis.

물리적 형태

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

기타 정보

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Nitric oxide release: Part II
Carpenter AW and Mark HS
Chemical Society Reviews, 41(10), 3742-3752 (2012)
Anjali Bajpai et al.
Developmental biology, 457(1), 1-8 (2019-09-27)
The Hedgehog (Hh) morphogen regulates growth and patterning. Since Hh signaling is also implicated in carcinogenesis, it is conceivable that de novo Hh-secreting organizers, if formed in association with oncogenic hit could be tumor-cooperative. Here we validate this hypothesis using

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